一种低成本便携式装置用于诱导小鼠无创性前交叉韧带损伤的验证。

IF 1.7 4区 医学 Q4 BIOPHYSICS
Elias H Jbeily, Yu-Yang Lin, Seif B Elmankabadi, Benjamin Osipov, Ron K June, Blaine A Christiansen
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引用次数: 0

摘要

无创压缩诱导的前交叉韧带断裂(ACL-R)是一种研究小鼠创伤后骨关节炎(PTOA)的简单且可重复的模型。然而,通常用于ACL-R的设备昂贵、不动,并且并非所有研究人员都能使用。在这项研究中,我们比较了用低成本定制ACL断裂装置(CARD)损伤的小鼠和用标准系统(ElectroForce 3200)损伤的鼠的PTOA进展。我们量化了损伤后即刻前后关节松弛度、骨骺小梁骨微观结构以及2和6时的骨赘体积 损伤后第2周和第6周使用微型计算机断层扫描,骨关节炎进展和滑膜炎 损伤后数周使用全关节组织学。我们观察到,与用电动力(ELF)系统损伤的小鼠相比,用CARD系统损伤的老鼠的结果没有显著差异。然而,AP关节松弛度数据和第2周的显微CT和组织学结果表明,与ELF系统相比,CARD系统损伤的小鼠的损伤可能稍微更严重,PTOA进展稍微更快。总之,这些数据证实了ACL-R可以用CARD系统成功且可重复地进行,骨关节炎(OA)的进展与用ELF系统损伤的小鼠的进展基本相当,尽管可能稍微快一点。CARD系统成本低且可移植,我们正在向所有感兴趣的研究人员免费提供计划和说明,希望他们能发现该系统对研究小鼠OA有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of a Low-Cost Portable Device for Inducing Noninvasive Anterior Cruciate Ligament Injury in Mice.

Noninvasive compression-induced anterior cruciate ligament rupture (ACL-R) is an easy and reproducible model for studying post-traumatic osteoarthritis (PTOA) in mice. However, equipment typically used for ACL-R is expensive, immobile, and not available to all researchers. In this study, we compared PTOA progression in mice injured with a low-cost custom ACL-rupture device (CARD) to mice injured with a standard system (ElectroForce 3200). We quantified anterior-posterior (AP) joint laxity immediately following injury, epiphyseal trabecular bone microstructure, and osteophyte volume at 2 and 6 weeks post injury using micro-computed tomography, and osteoarthritis progression and synovitis at 2 and 6 weeks post injury using whole-joint histology. We observed no significant differences in outcomes in mice injured with the CARD system compared to mice injured with the Electroforce (ELF) system. However, AP joint laxity data and week 2 micro-CT and histology outcomes suggested that injuries may have been slightly more severe and PTOA progressed slightly faster in mice injured with the CARD system compared to the ELF system. Altogether, these data confirm that ACL-R can be successfully and reproducibly performed with the CARD system and that osteoarthritis (OA) progression is mostly comparable to that of mice injured with the ELF system, though potentially slightly faster. The CARD system is low cost and portable, and we are making the plans and instructions freely available to all interested investigators in the hopes that they will find this system useful for their studies of OA in mice.

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来源期刊
CiteScore
3.40
自引率
5.90%
发文量
169
审稿时长
4-8 weeks
期刊介绍: Artificial Organs and Prostheses; Bioinstrumentation and Measurements; Bioheat Transfer; Biomaterials; Biomechanics; Bioprocess Engineering; Cellular Mechanics; Design and Control of Biological Systems; Physiological Systems.
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