猫脂肪组织间充质干细胞在 TNF-α 刺激下通过 COX-2/PGE2 激活改善 DSS 引起的小鼠结肠炎。

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Kyeongbo Kim, Ju-Hyun An, Su-Min Park, GaHyun Lim, Kyung-Won Seo, Hwa-Young Youn
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引用次数: 0

摘要

背景:间充质干细胞(MSCs)已被研究用作炎症性肠病(IBD)的治疗药物。用促炎细胞因子刺激间充质干细胞是增强其免疫调节作用的一种方法。然而,要支持间充质干细胞在免疫介导疾病和伴侣动物中的应用,还需要进一步的研究:本研究旨在评估肿瘤坏死因子(TNF)-α刺激的猫脂肪组织间充质干细胞(fAT-MSCs)在葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠模型中的治疗效果:方法:通过饮用含有 3% DSS 的水制造结肠炎小鼠,并腹腔注射 fAT-间充质干细胞。第 10 天收集结肠。对疾病的严重程度进行评估和比较。采用实时定量聚合酶链反应和酶联免疫吸附试验,用条件培养基培养原始 264.7 细胞,以确定其机制:结果:TNF-α刺激的fAT-间充质干细胞在疾病活动性、结肠长度、组织学评分和炎症细胞因子方面更能改善DSS诱导的结肠炎的严重程度。在结肠组织切片中,TNF-α刺激的fAT-MSCs组的CD11b+CD206+巨噬细胞比例高于其他组。在体外,TNF-α刺激可增加环氧化酶-2(COX-2)的表达和前列腺素E2(PGE2)的分泌。TNF-α刺激的fAT-间充质干细胞的条件培养基增强了LPS激活的Raw 264.7细胞中白细胞介素-10和精氨酸酶-1的表达:这些结果表明,TNF-α刺激的脂肪间充质干细胞能更有效地改善发炎的结肠。此外,我们还证明了这种有效性与 COX-2/PGE2 通路相互关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Amelioration of DSS-induced colitis in mice by TNF-α-stimulated mesenchymal stem cells derived from feline adipose tissue via COX-2/PGE<sub>2</sub> activation.

Amelioration of DSS-induced colitis in mice by TNF-α-stimulated mesenchymal stem cells derived from feline adipose tissue via COX-2/PGE<sub>2</sub> activation.

Amelioration of DSS-induced colitis in mice by TNF-α-stimulated mesenchymal stem cells derived from feline adipose tissue via COX-2/PGE<sub>2</sub> activation.

Amelioration of DSS-induced colitis in mice by TNF-α-stimulated mesenchymal stem cells derived from feline adipose tissue via COX-2/PGE2 activation.

Background: Mesenchymal stem cells (MSCs) have been investigated as therapeutic agents for inflammatory bowel disease (IBD). Stimulation of MSCs with pro-inflammatory cytokines is an approach to enhance their immunomodulatory effects. However, further investigation is required to support their application in immune-mediated disorders and companion animals.

Objectives: This study aimed to assess the therapeutic effect of tumor necrosis factor (TNF)-α-stimulated feline adipose tissue-derived MSCs (fAT-MSCs) in a dextran sulfate sodium (DSS)-induced colitis mouse model.

Methods: Colitis mice was made by drinking water with 3% DSS and fAT-MSCs were injected intraperitoneally. Colons were collected on day 10. The severity of the disease was evaluated and compared. Raw 264.7 cells were cultured with the conditioned medium to determine the mechanism, using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.

Results: TNF-α-stimulated fAT-MSCs more improved severity of DSS-induced colitis in disease activity, colon length, histologic score, and inflammatory cytokine. In sectionized colon tissues, the group comprising TNF-α-stimulated fAT-MSCs had higher proportion of CD11b+CD206+ macrophages than in the other groups. In vitro, TNF-α-stimulation increased cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) secretion from fAT-MSCs. The conditioned medium from TNF-α-stimulated fAT-MSCs enhanced the expression of interleukin-10 and arginase-1 in LPS-activated Raw 264.7 cells.

Conclusions: These results represent that TNF-α-stimulated fat-mscs ameliorate the inflamed colon more effectively. Furthermore, we demonstrated that the effectiveness was interlinked with the COX-2/PGE2 pathway.

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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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