费氏四目家兔多余B染色体与费氏四目家兔和鳗四目家兔标准染色体组重复序列相同。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Cytogenetic and Genome Research Pub Date : 2023-01-01 Epub Date: 2023-08-05 DOI:10.1159/000533431
Marina Souza Cunha, Camila Moura Novaes, Jaqueline Amorim Pereira, Martinha Mapingala Capoco, Tânia Maria Fernandes-Salomão, Denilce Meneses Lopes
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引用次数: 0

摘要

无刺蜜蜂Tetragonisca angustula和Tetragonisca fiebrigi广泛分布在巴西,两者通常被称为“jataí”。本研究的目的是研究fiebrigi T. B染色体的可能起源,这是区分fiebrigi T.和T. angustula的细胞分类特征。我们分析了二倍体染色体数目(2n)、B染色体发生率、组成异染色质模式以及不同重复DNA探针的原位定位。两种核型结构相似,均为2n = 34。只在赤柱中观察到1 ~ 3条B染色体。组成型异染色质分布在两种染色体的单臂上,赤杉B染色体以异染色质为主,一端为常染色质。(GA)15和(CAA)10微卫星探针标记了两种物种所有染色体的正染色臂,但未标记B染色体。18S核糖体DNA (rDNA)探针标记了巨齿弓形虫的10条染色体和fiebrigi的6条A染色体,并在3B个体中标记了1B染色体。Tan-Bsp68I重复DNA探针标记了所有费氏滴虫A和B染色体的异色部分。该探针还标记了所有舌鼠染色体的异色部分;因此,关于B染色体起源的两种假设都是可能的:(i)来自T. fifibrigi的A染色体补体(种内起源);或(ii) fiebrigi和T. angustula杂交后基因组重组的副产物(种间起源)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Supernumerary B Chromosomes of Tetragonisca fiebrigi Share Repeat Content with Standard Chromosome Set of both T. fiebrigi and Tetragonisca angustula (Apidae: Meliponini).

The stingless bees Tetragonisca angustula and Tetragonisca fiebrigi are widely distributed in Brazil, and both are commonly known as "jataí." Our goal was to investigate the possible origin of the B chromosomes in T. fiebrigi, a cytotaxonomic trait that differentiates T. fiebrigi from T. angustula. We analyzed diploid chromosome number (2n), B chromosome incidence, patterns of constitutive heterochromatin, and in situ localization of different repetitive DNA probes in T. angustula and T. fiebrigi. Both species displayed 2n = 34, with similar karyotype structures. One to three B chromosomes were observed in T. fiebrigi only. Constitutive heterochromatin was distributed on one arm of all chromosomes in both species, and T. fiebrigi B chromosomes were mainly heterochromatic with one euchromatic extremity. The (GA)15 and (CAA)10 microsatellite probes marked the euchromatic arms of all chromosomes in both species without marking the B chromosomes. The 18S ribosomal DNA (rDNA) probe marked 10 chromosomes in T. angustula and 6 A chromosomes in T. fiebrigi with an additional marking on 1B in individuals with 3B. The Tan-Bsp68I repetitive DNA probe marked the heterochromatic portion of all T. fiebrigi A and B chromosomes. This probe also marked the heterochromatic portion of all T. angustula chromosomes; therefore, both alternative hypotheses to the B chromosome origin are possible: (i) from the A chromosome complement of T. fiebrigi (intraspecific origin); or (ii) a by-product of genome reshuffling following the hybridization between T. fiebrigi and T. angustula (interspecific origin).

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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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