Vito A Damay, Setiawan, Ronny Lesmana, Muhammad Rizki Akbar, Antonia Anna Lukito, Vita M Tarawan, Januar W Martha, J Nugroho, Sony Sugiharto
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A total of 26 male Wistar rats (Rattus norvegicus) 8 weeks aged, 200-250 g b.w., were randomly divided into 4 groups, namely, K0 (normal rats), K1 (rats were given moderate-intensity aerobic exercise by animal treadmill 20 m/30 min), K2 (rats were given e-cigarette with 6 mg nicotine, 40% propylene glycol, and 60% vegetable glycerine 30 min for 5 days/week), and K3 (rats were given e-cigarette and moderate-intensity aerobic exercise). After exposure for 6 weeks, all animals were sacrificed to isolate the aorta for histopathological analysis with hematoxylin-eosin stain to evaluate the elastic fiber layer and intimal-medial thickness. The Verhoeff-Van Gieson staining was done for quantification elastic lamina fragmentation. Our study found that the e-cigarette group had the highest elastic lamina fragmentation among groups (8.14 ± 2.85). The exercise only group showed the lowest elastic lamina fragmentation (2.50 ± 1.87). Fragmentation in the e-cigarette and exercise group was higher than in the exercise only group (5.83 ± 0.753 vs. 2.50 ± 1.87, <i>p</i> = 0.002). There is a significant difference of NO serum between four groups. The result of post hoc analysis using LSD showed that there is a significant difference of NO serum between K0 and K2, K0 and K3, K1 and K2, and K1 and K3. Therefore, our research demonstrated that the most injury of aorta elastic lamina was in the group that was exposed to e-cigarette that leads to vascular aging while exercise is not yet proven to reverse this effect.</p>","PeriodicalId":14448,"journal":{"name":"International Journal of Vascular Medicine","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393525/pdf/","citationCount":"0","resultStr":"{\"title\":\"Aerobic Exercise versus Electronic Cigarette in Vascular Aging Process: First Histological Insight.\",\"authors\":\"Vito A Damay, Setiawan, Ronny Lesmana, Muhammad Rizki Akbar, Antonia Anna Lukito, Vita M Tarawan, Januar W Martha, J Nugroho, Sony Sugiharto\",\"doi\":\"10.1155/2023/8874599\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Smoking is related to vascular aging. 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引用次数: 0
摘要
吸烟与血管老化有关。然而,电子烟的有害影响往往是有争议的,可用的研究有限。相反,中等强度的有氧运动可以减少主动脉僵硬。我们提供了新的研究来确定电子烟和有氧中等强度运动对Wistar大鼠主动脉结构的影响。选取雄性褐家鼠(Rattus norvegicus) 26只,8周龄,200-250 g b.w.,随机分为4组,K0组(正常大鼠),K1组(在动物跑步机上进行20 m/30 min的中等强度有氧运动),K2组(给予尼古丁6 mg、40%丙二醇和60%植物甘油30 min的电子烟,连续5天/周),K3组(给予电子烟和中等强度有氧运动)。暴露6周后处死分离主动脉,用苏木精-伊红染色法进行组织病理学分析,评估弹性纤维层和内膜-内侧厚度。verhoefff - van Gieson染色定量测定弹性板断裂。我们的研究发现,电子烟组的弹性层裂率最高(8.14±2.85)。运动组弹性椎板碎裂率最低(2.50±1.87)。电子烟和运动组的碎片化程度高于运动组(5.83±0.753 vs. 2.50±1.87,p = 0.002)。四组间血清NO含量差异有统计学意义。LSD事后分析结果显示,血清NO在K0与K2、K0与K3、K1与K2、K1与K3之间存在显著差异。因此,我们的研究表明,主动脉弹性板损伤最严重的是电子烟暴露组,导致血管老化,而运动尚未被证明可以逆转这种影响。
Aerobic Exercise versus Electronic Cigarette in Vascular Aging Process: First Histological Insight.
Smoking is related to vascular aging. However, the hazardous effect of e-cigarette is often debatable, with limited studies available. In contrast, moderate-intensity aerobic exercise is well known to decrease aortic stiffness. We provide novel research to determine the effect of e-cigarette and aerobic moderate-intensity exercise on the aortic structure of Wistar rats. A total of 26 male Wistar rats (Rattus norvegicus) 8 weeks aged, 200-250 g b.w., were randomly divided into 4 groups, namely, K0 (normal rats), K1 (rats were given moderate-intensity aerobic exercise by animal treadmill 20 m/30 min), K2 (rats were given e-cigarette with 6 mg nicotine, 40% propylene glycol, and 60% vegetable glycerine 30 min for 5 days/week), and K3 (rats were given e-cigarette and moderate-intensity aerobic exercise). After exposure for 6 weeks, all animals were sacrificed to isolate the aorta for histopathological analysis with hematoxylin-eosin stain to evaluate the elastic fiber layer and intimal-medial thickness. The Verhoeff-Van Gieson staining was done for quantification elastic lamina fragmentation. Our study found that the e-cigarette group had the highest elastic lamina fragmentation among groups (8.14 ± 2.85). The exercise only group showed the lowest elastic lamina fragmentation (2.50 ± 1.87). Fragmentation in the e-cigarette and exercise group was higher than in the exercise only group (5.83 ± 0.753 vs. 2.50 ± 1.87, p = 0.002). There is a significant difference of NO serum between four groups. The result of post hoc analysis using LSD showed that there is a significant difference of NO serum between K0 and K2, K0 and K3, K1 and K2, and K1 and K3. Therefore, our research demonstrated that the most injury of aorta elastic lamina was in the group that was exposed to e-cigarette that leads to vascular aging while exercise is not yet proven to reverse this effect.