颞下颌关节纤维软骨含有CD105阳性小鼠间充质干/祖细胞,具有增加的软骨形成潜能。

IF 0.8 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
Journal of Maxillofacial & Oral Surgery Pub Date : 2023-09-01 Epub Date: 2022-05-09 DOI:10.1007/s12663-022-01721-6
Janis R Thamm, Youssef Jounaidi, Max-Laurin Mueller, Vicki Rosen, Maria J Troulis, Fernando Pozzi Semeghini Guastaldi
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引用次数: 0

摘要

目的:据报道,一种特殊类型的间充质干/祖细胞(MSPCs)CD105+通过TGF-β/Smad2信号传导帮助软骨再生。本研究的目的是鉴定和表征颞下颌关节(TMJ)软骨中CD105+MSPCs。材料和方法:从小鼠TMJ髁突外植体中分离出MSPCs,并对其克隆性和多潜能进行评估。应用免疫组织化学和流式细胞术检测MSPC的CD105抗原。结果:免疫组化显示髁突软骨增生区存在CD105+MSPCs。只有0.2%的分离的MSPCs表现出CD105以及干细胞表面标志物CD44和Sca-1。在CD105+MSPCs中,细胞内免疫染色显示显著更高(p p - 细胞,使用阿尔辛蓝染色。CD105+细胞与早期MSPC亚组相似,具有显著更高的二甘聚糖、蛋白多糖4、2型胶原、Gli2、Sox5基因表达(p p p p p - 细胞。结论:研究表明TMJ纤维软骨中存在CD105+MSPC亚群,该亚群可能被激活以帮助纤维软骨修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Temporomandibular Joint Fibrocartilage Contains CD105 Positive Mouse Mesenchymal Stem/Progenitor Cells with Increased Chondrogenic Potential.

Objective: A specific type of mesenchymal stem/progenitor cells (MSPCs), CD105+ is reported to aid in cartilage regeneration through TGF-β/Smad2-signalling. The purpose of this study was to identify and characterize CD105+ MSPCs in temporomandibular joint (TMJ) cartilage.

Materials and methods: MSPCs were isolated from mouse TMJ condyle explants and evaluated for their clonogenicity and pluripotential abilities. MSPC were examined for CD105 antigen using immunohistochemistry and flow cytometry.

Results: Immunohistochemistry revealed presence of CD105+ MSPCs in the proliferative zone of condyle's cartilage. Only 0.2% of isolated MSPCs exhibited CD105, along with the stem cell surface markers CD44 and Sca-1. In CD105+ MSPCs, intracellular immunostaining revealed significantly higher (p < 0.05) protein levels of collagen type 1, 2, proteoglycan 4. Ability for chondrogenic differentiation was found to be significantly higher (p < 0.05) after 4 weeks compared to CD105- cells, using alcian blue staining. CD105+ cells were found to resemble an early MSPC subgroup with significantly higher gene expression of biglycan, proteoglycan 4, collagen type 2, Gli2, Sox5 (p < 0.001) and Sox9 (p < 0.05). In contrast, significantly lower levels of Runx2 (p < 0.05), Osterix, Trps1, Col10a1 (p < 0.01), Ihh (p < 0.001) related to chondrocyte senescence and commitment to osteogenic lineage, were observed compared to CD105- cells.

Conclusion: The study showed the existence of a CD105+ MSPC subgroup within TMJ fibrocartilage that may be activated to aid in fibrocartilage repair.

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来源期刊
Journal of Maxillofacial & Oral Surgery
Journal of Maxillofacial & Oral Surgery DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
1.90
自引率
0.00%
发文量
138
期刊介绍: This journal offers comprehensive coverage of new techniques, important developments and innovative ideas in Oral and Maxillofacial Surgery. Practice-applicable articles help develop the methods used to handle dentoalveolar surgery, facial injuries and deformities, TMJ disorders, oral cancer, jaw reconstruction, anesthesia and analgesia. The journal also includes specifics on new instruments, diagnostic equipment’s and modern therapeutic drugs and devices. Journal of Oral and Maxillofacial Surgery is recommended for first or priority subscription by the Dental Section of the Medical Library Association. Specific topics covered recently have included: ? distraction osteogenesis ? synthetic bone substitutes ? fibroblast growth factors ? fetal wound healing ? skull base surgery ? computer-assisted surgery ? vascularized bone grafts Benefits to authorsWe also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services.
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