酰胺类局部麻醉剂对多种自由基的直接清除活性的量效关系。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Yukiko Sato, Shigekiyo Matsumoto, Kazue Ogata, Kira Bacal, Misato Nakatake, Takaaki Kitano, Osamu Tokumaru
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引用次数: 0

摘要

本研究旨在阐明酰胺基局麻药对体外多种自由基的直接清除活性的量效关系。我们已经证明了酰胺型局部麻醉剂选择性地和直接地清除一些自由基。四种局麻药均能消除三种自由基。甲哌卡因、利多卡因、布比卡因和二布卡因对羟基自由基的清除作用呈剂量依赖性。抗坏血酸自由基的清除也呈剂量依赖性,单线态氧的清除也呈剂量依赖性。另外三种自由基不能被所有四种局部麻醉剂清除;叔丁基自由基被所有的麻醉药清除,除了二布卡因,一氧化氮被甲哌卡因清除,但其他三种都没有,酪氨酸自由基被甲哌卡因和利多卡因清除,但其他两种都没有。部分自由基(超氧阴离子、叔丁基过氧基、DPPH)不能被四种局麻药清除。TBARS实验显示局麻药对脂质过氧化也有抑制作用。这些结果表明,局麻药通过清除自由基具有抗氧化作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals.

The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals.

The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals.

The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals.

This study aimed to illustrate the dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals in vitro. We have demonstrated that amide-type local anesthetics selectively and directly scavenge some free radicals. Three kinds of free radicals were eliminated by all the four local anesthetics examined. Mepivacaine, lidocaine, bupivacaine, and dibucaine scavenged hydroxyl radicals in dose-dependent manners. Ascorbyl free radicals were also scavenged in dose-dependent manners, and lastly singlet oxygen was scavenged in dose-dependent manners. Three other free radicals were not scavenged by all of the four local anesthetics; tert-butoxyl radical was scavenged by all the anesthetics examined but dibucaine, nitric oxide by mepivacaine but not by the other three, and tyrosyl radical by mepivacaine and lidocaine but not by the other two. Some free radicals (superoxide anion, tert-butyl peroxyl radical, DPPH) were not scavenged by any of the four local anesthetics. The local anesthetics were also shown to inhibit lipid peroxidation by TBARS assay. These results suggest that local anesthetics have antioxidant properties through their free radical scavenging activities.

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来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
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