Ali Pouryousef, Bahador Sarkari, Amir Mootabi Alavi, Mostafa Omidian, Fattaneh Mikaeili
{"title":"猫弓形虫第二阶段幼虫排泄-分泌抗原的去糖基化提高其对人类弓形虫病的诊断效果。","authors":"Ali Pouryousef, Bahador Sarkari, Amir Mootabi Alavi, Mostafa Omidian, Fattaneh Mikaeili","doi":"10.1155/2023/3024063","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Toxocariasis is an important zoonotic infection, especially in tropical areas. One of the significant challenges in the serodiagnosis of human toxocariasis is the cross-reaction of <i>Toxocara</i> antigens with other parasites due to their relatively similar glycan structures. Removing the glycan structure from <i>Toxocara</i> excretory-secretory (TES) antigens may increase the efficacy of these antigens in the diagnosis of toxocariasis. The current study aimed to assess the efficacy of deglycosylated <i>Toxocara cati</i> excretory-secretory (dTES) antigens for the serodiagnosis of human toxocariasis.</p><p><strong>Methods: </strong><i>Toxocara</i> ES antigens were prepared from <i>T. cati</i> second-stage larvae and deglycosylated using sodium hydroxide (NaOH). The TES antigens, along with the dTES antigens, were used in an ELISA as well as a western blotting system for the detection of anti-<i>Toxocara</i> antibodies. Sera samples collected from 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy subjects were evaluated by both systems.</p><p><strong>Results: </strong>The sensitivity of TES and dTES ELISA for the diagnosis of human toxocariasis was 96.67% (95% CI = 82.78-99.92) and 93.33% (95% CI = 77.93-99.18), respectively, while the specificity of dTES (88.33%; 95% CI = 77.43-95.18) increased significantly compared to the TES (80.00%; 95% CI = 67.67-89.22). The sensitivity of both antigens was 100% (95% CI = 88.43-100) by the western blotting system. Moreover, the specificity of TES and dTES antigens was 95% (95% CI = 86.08-98.96) and 98.33% (95% CI = 91.06-99.96), respectively, when using the western blotting system.</p><p><strong>Conclusion: </strong>Results of the current study indicate that the chemical removal of the glycan epitopes of <i>T. cati</i> ES antigens significantly reduces cross-reactivity rates with other parasitic infections. Considering the findings of the present study, the dTES antigens seem to be suitable antigens for the serodiagnosis of human toxocariasis.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"3024063"},"PeriodicalIF":2.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365917/pdf/","citationCount":"0","resultStr":"{\"title\":\"Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of <i>Toxocara cati</i> Improves Its Efficacy in the Diagnosis of Human Toxocariasis.\",\"authors\":\"Ali Pouryousef, Bahador Sarkari, Amir Mootabi Alavi, Mostafa Omidian, Fattaneh Mikaeili\",\"doi\":\"10.1155/2023/3024063\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Toxocariasis is an important zoonotic infection, especially in tropical areas. One of the significant challenges in the serodiagnosis of human toxocariasis is the cross-reaction of <i>Toxocara</i> antigens with other parasites due to their relatively similar glycan structures. Removing the glycan structure from <i>Toxocara</i> excretory-secretory (TES) antigens may increase the efficacy of these antigens in the diagnosis of toxocariasis. The current study aimed to assess the efficacy of deglycosylated <i>Toxocara cati</i> excretory-secretory (dTES) antigens for the serodiagnosis of human toxocariasis.</p><p><strong>Methods: </strong><i>Toxocara</i> ES antigens were prepared from <i>T. cati</i> second-stage larvae and deglycosylated using sodium hydroxide (NaOH). The TES antigens, along with the dTES antigens, were used in an ELISA as well as a western blotting system for the detection of anti-<i>Toxocara</i> antibodies. Sera samples collected from 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy subjects were evaluated by both systems.</p><p><strong>Results: </strong>The sensitivity of TES and dTES ELISA for the diagnosis of human toxocariasis was 96.67% (95% CI = 82.78-99.92) and 93.33% (95% CI = 77.93-99.18), respectively, while the specificity of dTES (88.33%; 95% CI = 77.43-95.18) increased significantly compared to the TES (80.00%; 95% CI = 67.67-89.22). The sensitivity of both antigens was 100% (95% CI = 88.43-100) by the western blotting system. Moreover, the specificity of TES and dTES antigens was 95% (95% CI = 86.08-98.96) and 98.33% (95% CI = 91.06-99.96), respectively, when using the western blotting system.</p><p><strong>Conclusion: </strong>Results of the current study indicate that the chemical removal of the glycan epitopes of <i>T. cati</i> ES antigens significantly reduces cross-reactivity rates with other parasitic infections. Considering the findings of the present study, the dTES antigens seem to be suitable antigens for the serodiagnosis of human toxocariasis.</p>\",\"PeriodicalId\":17527,\"journal\":{\"name\":\"Journal of Tropical Medicine\",\"volume\":\"2023 \",\"pages\":\"3024063\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365917/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Tropical Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1155/2023/3024063\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tropical Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2023/3024063","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
引用次数: 0
摘要
背景:弓形虫病是一种重要的人畜共患传染病，特别是在热带地区。人类弓形虫病血清诊断的重大挑战之一是弓形虫抗原与其他寄生虫的交叉反应，因为它们的聚糖结构相对相似。从弓形虫排泄-分泌(TES)抗原中去除多糖结构可能会提高这些抗原在弓形虫病诊断中的功效。本研究旨在评估去糖基化猫弓形虫排泄-分泌(dTES)抗原对人类弓形虫病的血清诊断效果。方法:从卡地弓形虫二期幼虫制备ES抗原，用氢氧化钠(NaOH)去糖基化。TES抗原和dTES抗原分别用于ELISA和western blotting系统检测抗弓形虫抗体。采集30例弓形虫病确诊病例、30例其他疾病患者和30名健康受试者的血清样本，采用这两种系统进行评估。结果:TES和dTES ELISA诊断人弓形虫病的灵敏度分别为96.67% (95% CI = 82.78 ~ 99.92)和93.33% (95% CI = 77.93 ~ 99.18)， dTES的特异性为88.33%;95% CI = 77.43-95.18)与TES (80.00%;95% ci = 67.67-89.22)。western blotting系统对两种抗原的敏感性均为100% (95% CI = 88.43 ~ 100)。此外，使用western blotting系统检测TES抗原和dTES抗原的特异性分别为95% (95% CI = 86.08-98.96)和98.33% (95% CI = 91.06-99.96)。结论:本研究结果表明，化学去除猫绦虫ES抗原的聚糖表位可显著降低其与其他寄生虫感染的交叉反应率。考虑到本研究的结果，dTES抗原似乎是人类弓形虫病血清诊断的合适抗原。
Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis.
Background: Toxocariasis is an important zoonotic infection, especially in tropical areas. One of the significant challenges in the serodiagnosis of human toxocariasis is the cross-reaction of Toxocara antigens with other parasites due to their relatively similar glycan structures. Removing the glycan structure from Toxocara excretory-secretory (TES) antigens may increase the efficacy of these antigens in the diagnosis of toxocariasis. The current study aimed to assess the efficacy of deglycosylated Toxocara cati excretory-secretory (dTES) antigens for the serodiagnosis of human toxocariasis.
Methods: Toxocara ES antigens were prepared from T. cati second-stage larvae and deglycosylated using sodium hydroxide (NaOH). The TES antigens, along with the dTES antigens, were used in an ELISA as well as a western blotting system for the detection of anti-Toxocara antibodies. Sera samples collected from 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy subjects were evaluated by both systems.
Results: The sensitivity of TES and dTES ELISA for the diagnosis of human toxocariasis was 96.67% (95% CI = 82.78-99.92) and 93.33% (95% CI = 77.93-99.18), respectively, while the specificity of dTES (88.33%; 95% CI = 77.43-95.18) increased significantly compared to the TES (80.00%; 95% CI = 67.67-89.22). The sensitivity of both antigens was 100% (95% CI = 88.43-100) by the western blotting system. Moreover, the specificity of TES and dTES antigens was 95% (95% CI = 86.08-98.96) and 98.33% (95% CI = 91.06-99.96), respectively, when using the western blotting system.
Conclusion: Results of the current study indicate that the chemical removal of the glycan epitopes of T. cati ES antigens significantly reduces cross-reactivity rates with other parasitic infections. Considering the findings of the present study, the dTES antigens seem to be suitable antigens for the serodiagnosis of human toxocariasis.
期刊介绍：
Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
