高强度间歇训练通过小鼠β3肾上腺素能受体改善肩袖脂肪浸润。

IF 4.7 2区 医学 Q2 CELL & TISSUE ENGINEERING
Hecheng Zhou, Chuanshun Chen, Hai Hu, Binbin Jiang, Yuesong Yin, Kexiang Zhang, Minren Shen, Song Wu, Zili Wang
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引用次数: 0

摘要

目的:肩袖肌萎缩和脂肪浸润影响肩袖撕裂患者的临床预后。然而,目前尚无有效的治疗脂肪浸润的方法。高强度间歇训练(HIIT)有助于激活米色脂肪组织。本研究的目的是测试HIIT通过β3肾上腺素能受体(β3AR)改善肩袖撕裂模型肌肉质量的作用。方法:3月龄C57BL/6 J小鼠单侧肩袖损伤。在肌腱撕裂手术后的第1 - 6周或第7 - 12周,小鼠被迫在跑步机上进行HIIT训练。为了研究β3AR的作用,我们在每次运动前10分钟通过腹腔注射选择性β3AR拮抗剂SR59230A。在肌腱撕裂后12周末收集冈上肌、肩胛间棕色脂肪和腹股沟皮下白色脂肪,并进行生物力学、组织学和生化分析。结果:冈上肌的组织学分析显示,与未运动组相比,HIIT改善了肌肉萎缩、脂肪浸润和收缩力。HIIT组冈上肌、肩胛间棕色脂肪和腹股沟皮下白色脂肪酪氨酸羟化酶和解偶联蛋白1表达增加,β3AR产热途径上调。然而,注射SR59230A的小鼠不存在HIIT的影响,这表明HIIT通过β3AR影响肌肉。结论:HIIT通过激活脂肪细胞附近的全身性交感神经纤维和β3AR,改善肩袖撕裂后的棘上肌质量和功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice.

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice.

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice.

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice.

Aims: Rotator cuff muscle atrophy and fatty infiltration affect the clinical outcomes of rotator cuff tear patients. However, there is no effective treatment for fatty infiltration at this time. High-intensity interval training (HIIT) helps to activate beige adipose tissue. The goal of this study was to test the role of HIIT in improving muscle quality in a rotator cuff tear model via the β3 adrenergic receptor (β3AR).

Methods: Three-month-old C57BL/6 J mice underwent a unilateral rotator cuff injury procedure. Mice were forced to run on a treadmill with the HIIT programme during the first to sixth weeks or seventh to 12th weeks after tendon tear surgery. To study the role of β3AR, SR59230A, a selective β3AR antagonist, was administered to mice ten minutes before each exercise through intraperitoneal injection. Supraspinatus muscle, interscapular brown fat, and inguinal subcutaneous white fat were harvested at the end of the 12th week after tendon tear and analyzed biomechanically, histologically, and biochemically.

Results: Histological analysis of supraspinatus muscle showed that HIIT improved muscle atrophy, fatty infiltration, and contractile force compared to the no exercise group. In the HIIT groups, supraspinatus muscle, interscapular brown fat, and inguinal subcutaneous white fat showed increased expression of tyrosine hydroxylase and uncoupling protein 1, and upregulated the β3AR thermogenesis pathway. However, the effect of HIIT was not present in mice injected with SR59230A, suggesting that HIIT affected muscles via β3AR.

Conclusion: HIIT improved supraspinatus muscle quality and function after rotator cuff tears by activating systemic sympathetic nerve fibre near adipocytes and β3AR.

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来源期刊
Bone & Joint Research
Bone & Joint Research CELL & TISSUE ENGINEERING-ORTHOPEDICS
CiteScore
7.40
自引率
23.90%
发文量
156
审稿时长
12 weeks
期刊介绍: The gold open access journal for the musculoskeletal sciences. Included in PubMed and available in PubMed Central.
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