Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso
{"title":"发现首个溶解性假中间葡萄球菌/金黄色葡萄球菌多价噬菌体。","authors":"Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso","doi":"10.1089/phage.2022.0004","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>There are no verified lytic <i>Staphylococcus pseudintermedius</i> phages in the literature and few temperate phage genomes in databases. <i>S. pseudintermedius</i> is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.</p><p><strong>Materials and methods: </strong>We discovered phages against canine-derived <i>S. pseudintermedius</i> isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.</p><p><strong>Results: </strong>Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are <i>S. pseudintermedius</i> and <i>Staphylococcus aureus</i> polyvalent phages, with DH2 and DS10 being strong killers of both species.</p><p><strong>Conclusions: </strong>We report discovery of the first verified lytic <i>S. pseudintermedius</i> phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-<i>Staphylococcal</i> phage cocktail.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 2","pages":"116-124"},"PeriodicalIF":0.0000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248872/pdf/phage.2022.0004.pdf","citationCount":"0","resultStr":"{\"title\":\"Discovery of the First Lytic <i>Staphylococcus pseudintermedius</i>/<i>Staphylococcus aureus</i> Polyvalent Bacteriophages.\",\"authors\":\"Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso\",\"doi\":\"10.1089/phage.2022.0004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>There are no verified lytic <i>Staphylococcus pseudintermedius</i> phages in the literature and few temperate phage genomes in databases. <i>S. pseudintermedius</i> is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.</p><p><strong>Materials and methods: </strong>We discovered phages against canine-derived <i>S. pseudintermedius</i> isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.</p><p><strong>Results: </strong>Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are <i>S. pseudintermedius</i> and <i>Staphylococcus aureus</i> polyvalent phages, with DH2 and DS10 being strong killers of both species.</p><p><strong>Conclusions: </strong>We report discovery of the first verified lytic <i>S. pseudintermedius</i> phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-<i>Staphylococcal</i> phage cocktail.</p>\",\"PeriodicalId\":74428,\"journal\":{\"name\":\"PHAGE (New Rochelle, N.Y.)\",\"volume\":\"3 2\",\"pages\":\"116-124\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248872/pdf/phage.2022.0004.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PHAGE (New Rochelle, N.Y.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/phage.2022.0004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/6/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2022.0004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/6/16 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Discovery of the First Lytic Staphylococcus pseudintermedius/Staphylococcus aureus Polyvalent Bacteriophages.
Background: There are no verified lytic Staphylococcus pseudintermedius phages in the literature and few temperate phage genomes in databases. S. pseudintermedius is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.
Materials and methods: We discovered phages against canine-derived S. pseudintermedius isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.
Results: Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are S. pseudintermedius and Staphylococcus aureus polyvalent phages, with DH2 and DS10 being strong killers of both species.
Conclusions: We report discovery of the first verified lytic S. pseudintermedius phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-Staphylococcal phage cocktail.
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