Two new lignan glycosides from Acanthus ilicifoliusL. with their NO inhibition and cytotoxic activity

A phytochemical investigation of the methanolic extract of aerial parts of the Acanthus ilicifolius led to the isolation of two new lignan glycosides, acaniliciosides A and B (1 and 2), together with ten known compounds (3-12). The structures of isolated compounds were elucidated based on HR-ESI-MS, 1D and 2D NMR spectroscopic data. The absolute configurations of two new compounds were established by CD spectra. With the exception of compound 12, other compounds inhibited NO production in LPS activated RAW264.7 cells with IC₅₀ values of 2.14-28.18 µM, as potent as that of the positive control of N^G-monomethyl-L-arginine acetate (L-NMMA, IC₅₀ of 32.50 µM). In addition, compounds 5-8 showed cytotoxic effects against SK-LU-1 and HepG2 cell lines with the IC₅₀ values ranging from 16.48 to 76.40 μM compared to the positive control (ellipticine) with the IC₅₀ values ranging from 1.23 to 1.46 μM.