酶引导自组装的多组分肽组装探索

IF 3.1 Q2 CHEMISTRY, MULTIDISCIPLINARY
Adrianna N. Shy, Jiashu Xu, Dr. Beom Jin Kim, Prof. Bing Xu
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引用次数: 1

摘要

基于主题(RNISY (M)和DEEVELILGDT (D))在梅林的蛋白质晶体结构和CRL4DCAF−1,我们磷酸化的酪氨酸残基米和共轭米一个自组装的主题产生此处则(1 p)和检查enzyme-instructed自组装(EISA) 1 p有或没有D(4)的存在。我们的结果表明,EISA 1 p形成水凝胶在非常低的体积分数(∼0.03%)即使有亲水性肽的存在,4。与1P不同的是,当2P (1P的非对映体)或3P (1P的对映体)的浓度分别为1P的4倍或3倍时,它们通过EISA形成水凝胶。圆二色性(CD)光谱表明,增加磷酸肽的浓度会降低混合物的CD信号,CD信号的大小取决于M和d之间的相互作用。这项工作为理解由自组装形成的多组分水凝胶提供了新的见解,其中包括特定的分子间相互作用和酶促反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An Exploration of Multiple Component Peptide Assemblies by Enzyme-Instructed Self-Assembly

An Exploration of Multiple Component Peptide Assemblies by Enzyme-Instructed Self-Assembly

Based on the motifs (RNISY (M) and DEEVELILGDT (D)) in the protein crystal structures of Merlin and CRL4DCAF−1, we phosphorylated the tyrosine residue in M and conjugated M to a self-assembling motif to produce a phosphopeptide (1P) and examined enzyme-instructed self-assembly (EISA) of 1P with and without the presence of D (4). Our results show that EISA of 1P forms a hydrogel at exceedingly low volume fraction (∼0.03 %) even with the presence of the hydrophilic peptide, 4. Unlike 1P, 2P (a diastereomer of 1P) or 3P (the enantiomer of 1P) forms a hydrogel via EISA when their concentration is four or three times that of 1P, respectively. Circular dichroism (CD) spectra show that increasing the concentration of the phosphopeptides lowers the CD signals of the mixtures, and the magnitudes of the CD signals depends on the interaction between M and D. This work provides insight for understanding multi-component hydrogels formed by self-assembly, which involves both specific intermolecular interaction and enzymatic reactions.

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CiteScore
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