使用Gabor滤波器集对小叶颈内腺增生进行判别分析:用Gabor滤波器特征对核不典型进行数值解释。

IF 1.6 4区 医学 Q3 PATHOLOGY
Acta Cytologica Pub Date : 2023-01-01 Epub Date: 2023-07-27 DOI:10.1159/000533255
Fumikazu Kimura, Kengo Ohshima, Keiichiro Shirai, Ryo Kanai, Masaki Sonohara, Keiko Ishii
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引用次数: 0

摘要

引言:小叶颈内腺增生(LEGH)是一种良性病变;然而,它被认为是子宫颈胃型腺癌的起源,早期诊断很重要。在神树大学医院,LEGH细胞的筛选是基于巴氏染色中细胞质粘蛋白色调的差异,并使用HIK1083标记的乳胶凝集试验检测胃粘蛋白。然而,有时很难区分具有细微核异型性的LEGH细胞和宫颈管(EC)细胞。方法:利用显微图像计算EC细胞(37例)和LEGH细胞(33例)细胞学标本细胞核的Gabor滤波器特征(平均信号值、标准差、偏度、峰度),并利用这些特征通过线性支持向量机(LSVM)进行统计分析和判别分析。Gabor滤波器是一种线性滤波器,定义为哺乳动物视觉系统的数学表示。使用具有三个波长和八个角度的Gabor滤波器进行分析。结果:EC细胞的Gabor滤波器特征高于LEGH细胞,表明LEGH细胞核的梯度比EC细胞核的梯度平缓。使用所有Gabor滤波器计算的精度为91.0%,四个Gabor滤波器(λ=2/3π和θ=0°,45°,90°,135°)的精度为88.9%。通过减少LSVM使用的特征数量,实现了高精度和低计算成本,而EC细胞的细胞是细而薄的。因此,认为EC细胞中像素突变梯度的频率高于LEGH细胞,并且LEGH细胞核中染色质分布的梯度比EC细胞核中的染色质分布梯度温和。通过使用Gabor滤波器量化细胞核的形态学特征,可以客观地评估EC和LEGH细胞的细胞核发现。使用Gabor滤波器特征使用LSVM可以将EC细胞与LEGH细胞区分开来。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Discriminant Analysis Using Gabor Filter Sets for Lobular Endocervical Glandular Hyperplasia: Numerical Interpretation of Nuclear Atypia by Gabor Filter Features.

Introduction: Lobular endocervical glandular hyperplasia (LEGH) is a benign lesion; however, it is considered to be the origin of gastric-type adenocarcinoma in the uterine cervix, and early diagnosis is important. At Shinshu University Hospital, screening of LEGH cells is based on the difference in color tone of cytoplasmic mucin on Papanicolaou staining and detection of gastric mucin using HIK1083-labeled latex agglutination assay. However, it is sometimes difficult to distinguish LEGH cells with subtle nuclear atypia from endocervical (EC) cells.

Methods: We calculated the Gabor filter features (mean signal value, standard deviation, skewness, kurtosis) from the nuclei of cytological specimens in EC cells (37 cases) and LEGH cells (33 cases) using microscopic images, and we performed statistical analysis and discriminant analysis by linear support vector machine (LSVM) using these features. A Gabor filter is a linear filter defined as a mathematical representation of the mammalian visual system. Gabor filters with three wavelengths and eight angles were used for analysis.

Results: Gabor filter features in EC cells were higher than in LEGH cells, demonstrating that the gradient of LEGH cell nuclei was milder than that of EC cell nuclei. The accuracy calculated using all Gabor filters was 91.0% and the accuracy of four Gabor filters (λ = 2/3π and θ = 0°, 45°, 90°, 135°) was 88.9%. High accuracy with low computation costs was achieved by reducing the number of features used for LSVM.

Conclusion: The application of a Gabor filter with convolutional processing resulted in the edges of LEGH cells being slightly rough and thick, whereas those of EC cells were fine and thin. Thus, it is thought that the frequency of abrupt gradients of pixels was higher in EC cells than in LEGH cells, and the gradient of chromatin distribution in LEGH cell nuclei was milder than that in EC cell nuclei. It was possible to evaluate nuclear findings of EC and LEGH cells objectively by quantifying morphological features of nuclei using Gabor filters. It was possible to differentiate EC cells from LEGH cells using LSVM using Gabor filter features.

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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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