在转基因猪到非人类灵长类动物模型中的辅助性异种肝移植技术:一种延长生存期的手术方法。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2023-09-01 Epub Date: 2023-07-26 DOI:10.1111/xen.12814
Kyo Won Lee, Sean S W Park, Dong Suk Kim, Kimyung Choi, Joohyun Shim, Jihun Kim, Sung Joo Kim, Jae Berm Park
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引用次数: 0

摘要

使用猪肝进行异种移植提供了一种潜在的替代方法,可以克服全球供体短缺的问题,或者更重要的是,作为同种异体移植的桥梁。然而,在非人类灵长类动物模型中,它受到了严重血小板减少症和致命凝血障碍的挑战。在这里,我们建议左侧辅助技术可以是一种有效的方法来延长异种移植物的存活时间。在猪-食蟹猴模型中进行了15次连续的异种肝脏移植。右辅助技术2例,原位辅助8例,左辅助5例。由于灵长类动物右叶和下腔静脉之间的复杂解剖过程中出血,手术后没有一名右侧辅助接受者存活下来。原位受试者因严重血小板减少和凝血障碍存活不到7天。五分之二的左侧辅助异种移植物存活超过3周,没有失控的血小板减少或贫血,其中一例存活34天,是迄今为止报道的最长移植物存活时间。在非人灵长类动物实验中,左侧辅助异种移植是一种可行的方法,可以将血小板减少和凝血障碍的风险降至最低。这可能允许对异种移植物进行更长时间的评估,并有助于更好地了解异种肝移植后发生的组织病理学和免疫学变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Auxiliary liver xenotransplantation technique in a transgenic pig-to-non-human primate model: A surgical approach to prolong survival.

Xenotransplantation using pigs' liver offers a potentially alternative method to overcome worldwide donor shortage, or more importantly as a bridge to allotransplantation. However, it has been challenged by profound thrombocytopenia and fatal coagulopathy in non-human primate models. Here we suggest that a left auxiliary technique can be a useful method to achieve extended survival of the xenograft. Fifteen consecutive liver xenotransplants were carried out in a pig-to-cynomolgus model. Right auxiliary technique was implemented in two cases, orthotopic in eight cases, and left auxiliary in five cases. None of the right auxiliary recipients survived after surgery due to hemorrhage during complex dissection between the primate's right lobe and inferior vena cava. Orthotopic recipients survived less than 7 days secondary to profound thrombocytopenia and coagulopathy. Two out of five left auxiliary xenotransplants survived more than 3 weeks without uncontrolled thrombocytopenia or anemia, with one of them surviving 34 days, the longest graft survival reported to date. Left auxiliary xenotransplant is a feasible approach in non-human primate experiments, and the feared risk of thrombocytopenia and coagulopathy can be minimized. This may allow for longer evaluation of the xenograft and help better understand histopathological and immunological changes that occur following liver xenotransplantation.

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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