尿路上皮去除不影响粘膜活性响应毒蕈碱或肾上腺素能受体的刺激。

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Christian Moro, Charlotte Phelps
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引用次数: 2

摘要

膀胱内膜(尿路上皮和固有层或膀胱粘膜)作为储存尿液和下层平滑肌之间的组织屏障,以及膀胱收缩性的调节和调节具有重要作用。然而,顶端尿路上皮层对该组织收缩活性的个别影响是不确定的。本实验的目的是确定尿路上皮切除后固有层的收缩活动。有几种方法被用来机械地破坏尿路上皮,包括用纸巾轻拍组织,用棉签纵向滑动,或者用手术刀的边缘纵向刮。苏木精-伊红染色测定顶端尿路上皮细胞的去除程度。在器官浴中测量了自发收缩活动,并获得了对激动剂萘醌和异丙肾上腺素的反应。用棉签纵向穿刺三次是去除大部分尿路上皮而不损伤固有层的最佳方法。除去尿路上皮后,组织的自发活性没有改变。同样,去除尿路上皮后,对甲氨基酚(1µM)和异丙肾上腺素(1µM)的反应不受影响。尿路上皮可以在不损伤固有层的情况下被有效移除。当毒蕈碱受体或肾上腺素能受体受到刺激时,这一根尖组织层不负责调节膀胱黏膜(固有层尿路上皮)自发性相活动或强直性收缩的增加。本研究认为固有层是调节膀胱壁整体收缩活动的重要细胞层。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Urothelium removal does not impact mucosal activity in response to muscarinic or adrenergic receptor stimulation.

The inner lining of the urinary bladder (urothelium and lamina propria, or bladder mucosa) has an important role as a tissue barrier between stored urine and the underlying smooth muscle, as well as in the modulation and regulation of bladder contractility. However, the individual influence of the apical urothelial layer on the contractile activity of this tissue is uncertain. The aim of this experiment was to identify the contractile activity of the lamina propria after removal of the urothelium. Several methods were used to mechanically disrupt the urothelium, including dabbing the tissue with a paper towel, longitudinal swipes with a cotton bud, or a longitudinal scrape with the edge of a scalpel. Hematoxylin-eosin staining was utilized to determine the level of removal of the apical urothelial cells. Spontaneous contractile activity was measured in organ baths, and responses to the agonists carbachol and isoprenaline were obtained. Three longitudinal swipes with a cotton bud was found to be the optimal method to remove the majority of the urothelium without damaging the lamina propria. Upon removal of the urothelium, the spontaneous activity of the tissue was unaltered. Similarly, responses to carbachol (1 µM) and isoprenaline (1 µM) were not affected after removal of the urothelium. The urothelium can be effectively removed without damaging the lamina propria. This apical tissue layer is not responsible for mediating the increases to spontaneous phasic activity or tonic contractions of the bladder mucosa (urothelium with lamina propria) when muscarinic or adrenergic receptors are stimulated. This research presents the lamina propria as the important cell layer mediating the overall contractile activity of the bladder wall.

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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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