(Tn5-) 3D/空间基因组学时代基于fish的成像。

Liheng Yang, Yan Yan, JunLin Li, Cheng Zhou, Jinlan Jin, Tongmei Zhang, Haokaifeng Wu, Xingang Li, Wei Wang, Li Yuan, Xu Zhang, Juntao Gao
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引用次数: 0

摘要

三维基因组学主要关注细胞水平上单个基因的三维位置,而空间基因组学更多关注组织水平。在这个令人兴奋的3D/空间基因组学新时代,已有半个世纪历史的FISH及其衍生方法,包括Tn5-FISH,发挥着重要作用。在这篇综述中,我们介绍了我们最近开发的Tn5-FISH,并介绍了我们和合作者发表的基于(Tn5-)FISH的六种不同的应用程序,这些应用程序可以是基于一般BAC克隆的FISH,也可以是Tn5-FISH。在这些有趣的案例中,(Tn5-)FISH显示出其针对不同疾病和细胞系(白血病、小鼠胚胎干细胞和分化细胞系)的亚染色体结构的强大能力。Tn5-FISH作为千碱基水平基因组结构成像的有效工具,在高通量检测染色体结构方面具有巨大的潜力,从而为3D/空间基因组学的伟大时代带来了新的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

(Tn5-)FISH-based imaging in the era of 3D/spatial genomics.

(Tn5-)FISH-based imaging in the era of 3D/spatial genomics.

(Tn5-)FISH-based imaging in the era of 3D/spatial genomics.

(Tn5-)FISH-based imaging in the era of 3D/spatial genomics.

3D genomics mainly focuses on the 3D position of single genes at the cell level, while spatial genomics focuses more on the tissue level. In this exciting new era of 3D/spatial genomics, half-century old FISH and its derivative methods, including Tn5-FISH, play important roles. In this review, we introduce the Tn5-FISH we developed recently, and present six different applications published by our collaborators and us, based on (Tn5-)FISH, which can be either general BAC clone-based FISH or Tn5-FISH. In these interesting cases, (Tn5-)FISH demonstrated its vigorous ability of targeting sub-chromosomal structures across different diseases and cell lines (leukemia, mESCs (mouse embryonic stem cells), and differentiation cell lines). Serving as an effective tool to image genomic structures at the kilobase level, Tn5-FISH holds great potential to detect chromosomal structures in a high-throughput manner, thus bringing the dawn for new discoveries in the great era of 3D/spatial genomics.

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CiteScore
1.30
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