Olga A. Podosokorskaya , Alexander G. Elcheninov , Andrei A. Novikov , Alexander Y. Merkel , Ilya V. Kublanov
{"title":"来自贝加尔湖地区温泉的嗜热水解细菌Fontisphaera persica gen.nov.，sp.nov.和Fontisphaeraceae fam的提议。nov.和Limisphaeraceae家族。11月，在Limisphaerales ord.nov.（Verrucomicrobiota）内。","authors":"Olga A. Podosokorskaya , Alexander G. Elcheninov , Andrei A. Novikov , Alexander Y. Merkel , Ilya V. Kublanov","doi":"10.1016/j.syapm.2023.126438","DOIUrl":null,"url":null,"abstract":"<div><p><span>A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 </span><sup>T</sup><span>, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30–57 °C and within a pH range of 5.1–8.4 with the optimum at 50 °C and pH 6.8–7.1. Strain B-154 </span><sup>T</sup><span><span><span><span> was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, </span>galactan, </span>galactomannan<span><span>, glucomannan<span>, xyloglucan, </span></span>pullulan<span>, arabinan, lichenan, beta-glucan, pachyman, </span></span></span>locust bean gum, xanthan gum). It did not require sodium chloride or yeast extract for growth. Major cellular fatty acids were anteiso-C</span><sub>15:0</sub>, <em>iso</em>-C<sub>16:0</sub> and <em>iso</em>-C<sub>14:0</sub><span>. The respiratory quinone was MK-7. The complete genome of strain B-154 </span><sup>T</sup><span> was 4.73 Mbp in size; its G + C content was 61%. According to the phylogenomic analysis strain B-154 </span><sup>T</sup><span> forms a separate family-level phylogenetic<span> lineage. Moreover, together with </span></span><em>Limisphaera ngatamarikiensis</em> and <em>“Pedosphaera parvula”</em> this strain forms a separate order-level phylogenetic lineage within <em>Verrucomicrobiae</em> class. Hence, we propose a novel order, <em>Limisphaerales</em> ord. nov., with two families <em>Limisphaeraceae</em> fam. nov. and <em>Fontisphaeraceae</em> fam. nov., and a novel genus and species <em>Fontisphaera persica</em> gen. nov., sp. nov. with type strain B-154 <sup>T</sup>. Ecogenomic analysis showed that representatives of the <em>Limisphaerales</em> are widespread in various environments. Although some of them were detected in hot springs the majority of <em>Limisphaerales</em><span> (54% of the studied metagenome-assembled genomes) were found in marine habitats. This study allowed a better understanding of physiology and ecology of </span><em>Verrucomicrobiota</em><span> – a rather understudied bacterial phylum.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126438"},"PeriodicalIF":3.3000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Fontisphaera persica gen. nov., sp. nov., a thermophilic hydrolytic bacterium from a hot spring of Baikal lake region, and proposal of Fontisphaeraceae fam. nov., and Limisphaeraceae fam. nov. within the Limisphaerales ord. nov. (Verrucomicrobiota)\",\"authors\":\"Olga A. Podosokorskaya , Alexander G. Elcheninov , Andrei A. Novikov , Alexander Y. Merkel , Ilya V. Kublanov\",\"doi\":\"10.1016/j.syapm.2023.126438\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 </span><sup>T</sup><span>, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30–57 °C and within a pH range of 5.1–8.4 with the optimum at 50 °C and pH 6.8–7.1. Strain B-154 </span><sup>T</sup><span><span><span><span> was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, </span>galactan, </span>galactomannan<span><span>, glucomannan<span>, xyloglucan, </span></span>pullulan<span>, arabinan, lichenan, beta-glucan, pachyman, </span></span></span>locust bean gum, xanthan gum). It did not require sodium chloride or yeast extract for growth. Major cellular fatty acids were anteiso-C</span><sub>15:0</sub>, <em>iso</em>-C<sub>16:0</sub> and <em>iso</em>-C<sub>14:0</sub><span>. The respiratory quinone was MK-7. The complete genome of strain B-154 </span><sup>T</sup><span> was 4.73 Mbp in size; its G + C content was 61%. According to the phylogenomic analysis strain B-154 </span><sup>T</sup><span> forms a separate family-level phylogenetic<span> lineage. Moreover, together with </span></span><em>Limisphaera ngatamarikiensis</em> and <em>“Pedosphaera parvula”</em> this strain forms a separate order-level phylogenetic lineage within <em>Verrucomicrobiae</em> class. Hence, we propose a novel order, <em>Limisphaerales</em> ord. nov., with two families <em>Limisphaeraceae</em> fam. nov. and <em>Fontisphaeraceae</em> fam. nov., and a novel genus and species <em>Fontisphaera persica</em> gen. nov., sp. nov. with type strain B-154 <sup>T</sup>. Ecogenomic analysis showed that representatives of the <em>Limisphaerales</em> are widespread in various environments. Although some of them were detected in hot springs the majority of <em>Limisphaerales</em><span> (54% of the studied metagenome-assembled genomes) were found in marine habitats. This study allowed a better understanding of physiology and ecology of </span><em>Verrucomicrobiota</em><span> – a rather understudied bacterial phylum.</span></p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"46 4\",\"pages\":\"Article 126438\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000474\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000474","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Fontisphaera persica gen. nov., sp. nov., a thermophilic hydrolytic bacterium from a hot spring of Baikal lake region, and proposal of Fontisphaeraceae fam. nov., and Limisphaeraceae fam. nov. within the Limisphaerales ord. nov. (Verrucomicrobiota)
A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 T, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30–57 °C and within a pH range of 5.1–8.4 with the optimum at 50 °C and pH 6.8–7.1. Strain B-154 T was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, galactan, galactomannan, glucomannan, xyloglucan, pullulan, arabinan, lichenan, beta-glucan, pachyman, locust bean gum, xanthan gum). It did not require sodium chloride or yeast extract for growth. Major cellular fatty acids were anteiso-C15:0, iso-C16:0 and iso-C14:0. The respiratory quinone was MK-7. The complete genome of strain B-154 T was 4.73 Mbp in size; its G + C content was 61%. According to the phylogenomic analysis strain B-154 T forms a separate family-level phylogenetic lineage. Moreover, together with Limisphaera ngatamarikiensis and “Pedosphaera parvula” this strain forms a separate order-level phylogenetic lineage within Verrucomicrobiae class. Hence, we propose a novel order, Limisphaerales ord. nov., with two families Limisphaeraceae fam. nov. and Fontisphaeraceae fam. nov., and a novel genus and species Fontisphaera persica gen. nov., sp. nov. with type strain B-154 T. Ecogenomic analysis showed that representatives of the Limisphaerales are widespread in various environments. Although some of them were detected in hot springs the majority of Limisphaerales (54% of the studied metagenome-assembled genomes) were found in marine habitats. This study allowed a better understanding of physiology and ecology of Verrucomicrobiota – a rather understudied bacterial phylum.
期刊介绍：
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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