分子伴侣HSP40、HSP70、STIP1和HSP90参与Cx43的稳定。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2023-06-01 Epub Date: 2023-02-03 DOI:10.1007/s10616-023-00570-6
Li An, Hong Gao, Yi Zhong, Yanqiu Liu, Ying Cao, Jing Yi, Xiang Huang, Chunlei Wen, Rui Tong, Zhijun Pan, Xu Yan, Meiyan Liu, Shengzhao Wang, Xue Bai, Hao Wu, Tingju Hu
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引用次数: 0

摘要

探讨应激诱导的磷蛋白1(STIP1)、热休克蛋白(HSP)70和HSP90在大鼠H9c2心肌细胞连接蛋白43(Cx43)泛素化中的作用。免疫共沉淀法用于检测蛋白质-蛋白质相互作用和Cx43泛素化。免疫荧光用于蛋白质共定位。在STIP1和/或HSP90表达修饰的H9c2细胞中重新分析蛋白质结合、Cx43蛋白表达和Cx43泛素化。在正常H9c2心肌细胞中,STIP1与HSP70和HSP90结合,Cx43与HSP40、HSP70和HSP90结合。STIP1的过表达促进了Cx43-HSP70向Cx43-HSP90的转变,并抑制了Cx43的泛素化;STIP1的敲除导致了相反的效果。HSP90的抑制抵消了STIP1过表达对Cx43泛素化的抑制作用。STIP1通过促进Cx43-HSP70向Cx43-HSP90的转变来抑制H9c2心肌细胞中Cx43的泛素化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Molecular chaperones HSP40, HSP70, STIP1, and HSP90 are involved in stabilization of Cx43.

Molecular chaperones HSP40, HSP70, STIP1, and HSP90 are involved in stabilization of Cx43.

To investigate the involvement of stress induced phosphoprotein 1 (STIP1), heat shock protein (HSP) 70, and HSP90 in ubiquitination of connexin 43 (Cx43) in rat H9c2 cardiomyocytes. Co-immunoprecipitation was used to detect protein-protein interactions and Cx43 ubiquitination. Immunofluorescence was used for protein co-localization. The protein binding, Cx43 protein expression, and Cx43 ubiquitination were reanalyzed in H9c2 cells with modified STIP1 and/or HSP90 expression. STIP1 bound to HSP70 and HSP90, and Cx43 bound to HSP40, HSP70, and HSP90 in normal H9c2 cardiomyocytes. Overexpression of STIP1 promoted the transition of Cx43-HSP70 to Cx43-HSP90 and inhibited Cx43 ubiquitination; knockdown of STIP1 resulted in the opposite effects. Inhibition of HSP90 counteracted the inhibitory effect of STIP1 overexpression on Cx43 ubiquitination. STIP1 suppresses Cx43 ubiquitination in H9c2 cardiomyocytes by promoting the transition of Cx43-HSP70 to Cx43-HSP90.

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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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