化学合成CuO-NPs和MgO-NPs防治马铃薯褐腐病

IF 3.4 3区 生物学 Q1 Agricultural and Biological Sciences
Amira Rabea, E Naeem, Naglaa M Balabel, Ghadir E Daigham
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引用次数: 1

摘要

背景:就生产和消费而言,土豆是埃及重要的蔬菜作物。然而,马铃薯业每年因褐腐病遭受重大损失。本研究旨在利用高效经济的CuO和MgO纳米金属氧化物等药物,在体外和体内抑制马铃薯褐腐病病原Ralstonia solanacearum (R. solanacearum),以降低农药残留风险。结果:用简单的化学方法合成了氧化铜和氧化镁纳米颗粒。利用紫外可见光谱、透射电子显微镜(TEM)、zeta电位分析、x射线衍射(XRD)和傅里叶变换红外光谱(FTIR)对纳米颗粒的平均粒径、形貌和结构进行了表征。CuO和MgO NPs浓度为3 mg/mL时,对茄青的生长有较强的抑制作用,抑制区(ZOI)分别为19.3 mm和17 mm。CuO-NPs和MgO-NPs的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为0.5、0.6和0.6、0.75 mg/mL。在体内施用不同MIC浓度的CuO-NPs和MgO-NPs时,褐腐病发病率分别显著降低至71.2%和69.4%,而散装CuSO4和散装MgSO4处理分别为43.0%和39.5%。与对照植株相比,CuO-NPs和MgO-NPs显著提高了马铃薯植株的产量、总叶绿素含量和酶效率。透射电镜显示,细菌细胞膜在与CuO-NPs和MgO-NPs相互作用后受到纳米力学力的严重破坏,这一点得到了脂质过氧化和超微结构研究的证实。结论:CuO-NPs和MgO-NPs可作为农业植物病原体管理的智能代理。使用金属氧化物纳米粒子可以为治疗植物病害提供一种无风险的替代方法,而植物病害是埃及马铃薯产业目前面临的最大挑战之一。与受感染的对照植株相比,经CuO-NPs和MgO-NPs处理的马铃薯植株在产量、光合色素、酶活性和总酚促进下对茄枯病菌的抗性均显著提高,这凸显了埃及马铃薯产业的潜在效益。利用金属氧化物纳米颗粒治疗其他植物病害还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Management of potato brown rot disease using chemically synthesized CuO-NPs and MgO-NPs.

Management of potato brown rot disease using chemically synthesized CuO-NPs and MgO-NPs.

Management of potato brown rot disease using chemically synthesized CuO-NPs and MgO-NPs.

Management of potato brown rot disease using chemically synthesized CuO-NPs and MgO-NPs.

Background: Potatoes are a crucial vegetable crop in Egypt in terms of production and consumption. However, the potato industry suffers significant annual losses due to brown rot disease. This study aimed to suppress Ralstonia solanacearum (R. solanacearum), the causative agent of brown rot disease in potatoes, using efficient and economical medications such as CuO and MgO metal oxide nanoparticles, both in vitro and in vivo, to reduce the risk of pesticide residues.

Results: CuO and MgO metal oxide nanoparticles were synthesized via a simple chemical process. The average particle size, morphology, and structure of the nanoparticles were characterized using UV-visible spectroscopy, transmission electron microscopy (TEM), zeta potential analysis, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The growth of R. solanacearum was strongly inhibited by CuO and MgO NPs at a concentration of 3 mg/mL, resulting in zones of inhibition (ZOI) of 19.3 mm and 17 mm, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CuO-NPs and MgO-NPs were 0.5, 0.6, and 0.6, 0.75 mg/mL, respectively. When applied in vivo through seed dressing and tuber soaking at their respective MIC concentrations, CuO-NPs and MgO-NPs significantly reduced the incidence of brown rot disease to 71.2% and 69.4%, respectively, compared to 43.0% and 39.5% in bulk CuSO4 and bulk MgSO4 treatments, respectively. Furthermore, CuO-NPs and MgO-NPs significantly increased the yield, total chlorophyll content, and enzyme efficiency of potato plants compared with the infected control plants. TEM revealed that the bacterial cytomembrane was severely damaged by nanomechanical forces after interaction with CuO-NPs and MgO-NPs, as evidenced by lipid peroxidation and ultrastructural investigations.

Conclusion: The results of this study suggest that CuO-NPs and MgO-NPs can be used as intelligent agents to manage plant pathogens in agriculture. The use of metal oxide nanoparticles could provide a risk-free alternative for treating plant diseases, which are currently one of the biggest challenges faced by the potato industry in Egypt. The significant increase in yield, photosynthetic pigments, enzymatic activity, and total phenol-promoted resistance to R. solanacearum in potato plants treated with CuO-NPs and MgO-NPs compared to infected control plants highlights the potential benefits for the potato industry in Egypt. Further investigations are needed to explore using metal oxide nanoparticles for treating other plant diseases.

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来源期刊
Botanical Studies
Botanical Studies 生物-植物科学
CiteScore
5.50
自引率
2.90%
发文量
32
审稿时长
2.4 months
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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