Nasrin Amirrajab, Seyed Amin Ayatollahi Mousavi, Behrooz Taheri, Samira Salari, Noor Ali Ahmadi Sarsahra
{"title":"摩罗菌素样肽对白色念珠菌、光秃念珠菌和热带念珠菌体外抗真菌效力的研究。","authors":"Nasrin Amirrajab, Seyed Amin Ayatollahi Mousavi, Behrooz Taheri, Samira Salari, Noor Ali Ahmadi Sarsahra","doi":"10.18502/ijm.v15i3.12907","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The aim of this study was to investigate the <i>in vitro</i> antifungal potency of the moronecidin-like peptide against <i>Candida albicans, Candida glabrata</i>, and <i>Candida tropicalis.</i></p><p><strong>Materials and methods: </strong>To evaluate the antifungal effect of moronecidin-like peptide, the protocol presented in CLSI M27-A3 and CLSI M27-S4 was used and the minimum inhibitory concentration was determined.</p><p><strong>Results: </strong>The minimum inhibitory effect of moronecidin-like peptide composition was 8 µg/ml for <i>Candida tropicalis</i> and <i>Candida albicans</i> and 32 µg/ml for <i>Candida glabrata</i>. The MIC of nystatin was determined to be 1.25 µg/ml for <i>Candida glabrata</i> and <i>Candida albicans</i> and 0.625 µg/ml for <i>Candida tropicalis</i> strains. The MFC composition of the moronecidin-like peptide was determined for <i>Candida tropicalis</i> and <i>Candida albicans</i> strains 8 µg/ml and for <i>Candida glabrata</i> strain 64 µg/ml. The results of cytotoxicity and hemolysis of the moronecidin peptide test on macrophage showed that moronecidin peptide has no cytotoxicity and toxicity properties.</p><p><strong>Conclusion: </strong>According to the results of the present study, the moronecidin-like peptide could be a new strategy in the treatment of infections caused by <i>Candida</i> strains. The discovery of the exact mechanism of which requires extensive clinical studies in this field.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"456-461"},"PeriodicalIF":1.3000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/c9/IJM-15-456.PMC10336287.pdf","citationCount":"0","resultStr":"{\"title\":\"<i>In vitro</i> antifungal potency of the moronecidin-like peptide against <i>Candida albicans, Candida glabrata,</i> and <i>Candida tropicalis</i>.\",\"authors\":\"Nasrin Amirrajab, Seyed Amin Ayatollahi Mousavi, Behrooz Taheri, Samira Salari, Noor Ali Ahmadi Sarsahra\",\"doi\":\"10.18502/ijm.v15i3.12907\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>The aim of this study was to investigate the <i>in vitro</i> antifungal potency of the moronecidin-like peptide against <i>Candida albicans, Candida glabrata</i>, and <i>Candida tropicalis.</i></p><p><strong>Materials and methods: </strong>To evaluate the antifungal effect of moronecidin-like peptide, the protocol presented in CLSI M27-A3 and CLSI M27-S4 was used and the minimum inhibitory concentration was determined.</p><p><strong>Results: </strong>The minimum inhibitory effect of moronecidin-like peptide composition was 8 µg/ml for <i>Candida tropicalis</i> and <i>Candida albicans</i> and 32 µg/ml for <i>Candida glabrata</i>. The MIC of nystatin was determined to be 1.25 µg/ml for <i>Candida glabrata</i> and <i>Candida albicans</i> and 0.625 µg/ml for <i>Candida tropicalis</i> strains. The MFC composition of the moronecidin-like peptide was determined for <i>Candida tropicalis</i> and <i>Candida albicans</i> strains 8 µg/ml and for <i>Candida glabrata</i> strain 64 µg/ml. The results of cytotoxicity and hemolysis of the moronecidin peptide test on macrophage showed that moronecidin peptide has no cytotoxicity and toxicity properties.</p><p><strong>Conclusion: </strong>According to the results of the present study, the moronecidin-like peptide could be a new strategy in the treatment of infections caused by <i>Candida</i> strains. The discovery of the exact mechanism of which requires extensive clinical studies in this field.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"15 3\",\"pages\":\"456-461\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/c9/IJM-15-456.PMC10336287.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v15i3.12907\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v15i3.12907","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
In vitro antifungal potency of the moronecidin-like peptide against Candida albicans, Candida glabrata, and Candida tropicalis.
Background and objectives: The aim of this study was to investigate the in vitro antifungal potency of the moronecidin-like peptide against Candida albicans, Candida glabrata, and Candida tropicalis.
Materials and methods: To evaluate the antifungal effect of moronecidin-like peptide, the protocol presented in CLSI M27-A3 and CLSI M27-S4 was used and the minimum inhibitory concentration was determined.
Results: The minimum inhibitory effect of moronecidin-like peptide composition was 8 µg/ml for Candida tropicalis and Candida albicans and 32 µg/ml for Candida glabrata. The MIC of nystatin was determined to be 1.25 µg/ml for Candida glabrata and Candida albicans and 0.625 µg/ml for Candida tropicalis strains. The MFC composition of the moronecidin-like peptide was determined for Candida tropicalis and Candida albicans strains 8 µg/ml and for Candida glabrata strain 64 µg/ml. The results of cytotoxicity and hemolysis of the moronecidin peptide test on macrophage showed that moronecidin peptide has no cytotoxicity and toxicity properties.
Conclusion: According to the results of the present study, the moronecidin-like peptide could be a new strategy in the treatment of infections caused by Candida strains. The discovery of the exact mechanism of which requires extensive clinical studies in this field.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
