Tomeu Viver , Roth E. Conrad , Marianna Lucio , Mourad Harir , Mercedes Urdiain , Juan F. Gago , Ana Suárez-Suárez , Esteban Bustos-Caparros , Rodrigo Sanchez-Martinez , Eva Mayol , Federico Fassetta , Jinfeng Pang , Ionuț Mădălin Gridan , Stephanus Venter , Fernando Santos , Bonnie Baxter , María E. Llames , Adorján Cristea , Horia L. Banciu , Brian P. Hedlund , Ramon Rossello-Mora
{"title":"两个已培育和两个未培育的新Salinibacter物种的描述，其中一个按照细菌编码规则命名：Salinibactergrassmerensis sp.nov。；三个按照SeqCode规则命名：Salinibacter pepae sp.nov.、Salinibacter-abssi sp.nov..和Salinibacer pampae sp.nov。","authors":"Tomeu Viver , Roth E. Conrad , Marianna Lucio , Mourad Harir , Mercedes Urdiain , Juan F. Gago , Ana Suárez-Suárez , Esteban Bustos-Caparros , Rodrigo Sanchez-Martinez , Eva Mayol , Federico Fassetta , Jinfeng Pang , Ionuț Mădălin Gridan , Stephanus Venter , Fernando Santos , Bonnie Baxter , María E. Llames , Adorján Cristea , Horia L. Banciu , Brian P. Hedlund , Ramon Rossello-Mora","doi":"10.1016/j.syapm.2023.126416","DOIUrl":null,"url":null,"abstract":"<div><p>Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new <em>Salinibacter</em> species. For these species we propose the names <em>Salinibacter pepae</em> sp. nov. and <em>Salinibacter grassmerensis</em> sp. nov. (showing average nucleotide identity (ANI) values < 95.09% and 87.08% with <em>Sal. ruber</em> M31<sup>T</sup>, respectively). Metabolomics revealed species-specific discriminative profiles. <em>Sal. ruber</em> strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and <em>Sal. altiplanensis</em> was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus <em>Salinibacter</em>. These species dominated in different sites and always coexisted with <em>Sal. ruber</em> and <em>Sal. pepae.</em> Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species <em>Salinibacter pampae</em> sp. nov. and <em>Salinibacter abyssi</em> sp. nov. respectively (showing ANI values 90.94% and 91.48% with <em>Sal. ruber</em> M31<sup>T</sup>, respectively). <em>Sal. grassmerensis</em> sp. nov. name was formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP), and <em>Sal. pepae</em>, <em>Sal. pampae</em> sp. nov. and <em>Sal. abyssi</em> sp. nov. are proposed following the rules of the newly published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist, and how the official naming a cultivated organism for which the deposit in public repositories is difficult finds an intermediate solution.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov.\",\"authors\":\"Tomeu Viver , Roth E. Conrad , Marianna Lucio , Mourad Harir , Mercedes Urdiain , Juan F. Gago , Ana Suárez-Suárez , Esteban Bustos-Caparros , Rodrigo Sanchez-Martinez , Eva Mayol , Federico Fassetta , Jinfeng Pang , Ionuț Mădălin Gridan , Stephanus Venter , Fernando Santos , Bonnie Baxter , María E. Llames , Adorján Cristea , Horia L. Banciu , Brian P. Hedlund , Ramon Rossello-Mora\",\"doi\":\"10.1016/j.syapm.2023.126416\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new <em>Salinibacter</em> species. For these species we propose the names <em>Salinibacter pepae</em> sp. nov. and <em>Salinibacter grassmerensis</em> sp. nov. (showing average nucleotide identity (ANI) values < 95.09% and 87.08% with <em>Sal. ruber</em> M31<sup>T</sup>, respectively). Metabolomics revealed species-specific discriminative profiles. <em>Sal. ruber</em> strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and <em>Sal. altiplanensis</em> was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus <em>Salinibacter</em>. These species dominated in different sites and always coexisted with <em>Sal. ruber</em> and <em>Sal. pepae.</em> Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species <em>Salinibacter pampae</em> sp. nov. and <em>Salinibacter abyssi</em> sp. nov. respectively (showing ANI values 90.94% and 91.48% with <em>Sal. ruber</em> M31<sup>T</sup>, respectively). <em>Sal. grassmerensis</em> sp. nov. name was formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP), and <em>Sal. pepae</em>, <em>Sal. pampae</em> sp. nov. and <em>Sal. abyssi</em> sp. nov. are proposed following the rules of the newly published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist, and how the official naming a cultivated organism for which the deposit in public repositories is difficult finds an intermediate solution.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000255\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000255","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov.
Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new Salinibacter species. For these species we propose the names Salinibacter pepae sp. nov. and Salinibacter grassmerensis sp. nov. (showing average nucleotide identity (ANI) values < 95.09% and 87.08% with Sal. ruber M31T, respectively). Metabolomics revealed species-specific discriminative profiles. Sal. ruber strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and Sal. altiplanensis was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus Salinibacter. These species dominated in different sites and always coexisted with Sal. ruber and Sal. pepae. Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species Salinibacter pampae sp. nov. and Salinibacter abyssi sp. nov. respectively (showing ANI values 90.94% and 91.48% with Sal. ruber M31T, respectively). Sal. grassmerensis sp. nov. name was formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP), and Sal. pepae, Sal. pampae sp. nov. and Sal. abyssi sp. nov. are proposed following the rules of the newly published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist, and how the official naming a cultivated organism for which the deposit in public repositories is difficult finds an intermediate solution.
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