乳腺癌启动子甲基化调控的差异表达基因

IF 3.3 4区 医学 Q2 ONCOLOGY
Samar Sindi, Norah Hamdi, Sabah Hassan, Magdah Ganash, Mona Alharbi, Najla Alburae, Sheren Azhari, Shadi Alkhayyat, Ayman Linjawi, Heba Alkhatabi, Aisha Elaimi, Ghadeer Alrefaei, Nouf Alsubhi, Aziza Alrafiah, Safiah Alhazmi
{"title":"乳腺癌启动子甲基化调控的差异表达基因","authors":"Samar Sindi,&nbsp;Norah Hamdi,&nbsp;Sabah Hassan,&nbsp;Magdah Ganash,&nbsp;Mona Alharbi,&nbsp;Najla Alburae,&nbsp;Sheren Azhari,&nbsp;Shadi Alkhayyat,&nbsp;Ayman Linjawi,&nbsp;Heba Alkhatabi,&nbsp;Aisha Elaimi,&nbsp;Ghadeer Alrefaei,&nbsp;Nouf Alsubhi,&nbsp;Aziza Alrafiah,&nbsp;Safiah Alhazmi","doi":"10.2147/BCTT.S408711","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is one of the most common malignancies among women. Recent studies revealed that differentially methylated regions (DMRs) are implicated in regulating gene expression. The goal of this research was to determine which genes and pathways are dysregulated in breast cancer when their promoters are methylated in an abnormal way, leading to differential expression. Whole-genome bisulfite sequencing was applied to analyze DMRs for eight peripheral blood samples collected from five Saudi females diagnosed with stages I and II of breast cancer aligned with three normal females. Three of those patients and three normal samples were used to determine differentially expressed genes (DEG) using Illumina platform NovaSeq PE150.</p><p><strong>Results: </strong>Based on ontology (GO) and KEGG pathways, the analysis indicated that DMGs and DEG are closely related to associated processes, such as ubiquitin-protein transferase activity, ubiquitin-mediated proteolysis, and oxidative phosphorylation. The findings indicated a potentially significant association between global hypomethylation and breast cancer in Saudi patients. Our results revealed 81 differentially promoter-methylated and expressed genes. The most significant differentially methylated and expressed genes found in gene ontology (GO) are pumilio RNA binding family member 1 (<i>PUM1</i>) and zinc finger AN1-type containing 2B (<i>ZFAND2B</i>) also known as (<i>AIRAPL</i>).</p><p><strong>Conclusion: </strong>The essential outcomes of this study suggested that aberrant hypermethylation at crucial genes that have significant parts in the molecular pathways of breast cancer could be used as a potential prognostic biomarker for breast cancer.</p>","PeriodicalId":9106,"journal":{"name":"Breast Cancer : Targets and Therapy","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fa/e0/bctt-15-435.PMC10332364.pdf","citationCount":"0","resultStr":"{\"title\":\"Promoter Methylation-Regulated Differentially Expressed Genes in Breast Cancer.\",\"authors\":\"Samar Sindi,&nbsp;Norah Hamdi,&nbsp;Sabah Hassan,&nbsp;Magdah Ganash,&nbsp;Mona Alharbi,&nbsp;Najla Alburae,&nbsp;Sheren Azhari,&nbsp;Shadi Alkhayyat,&nbsp;Ayman Linjawi,&nbsp;Heba Alkhatabi,&nbsp;Aisha Elaimi,&nbsp;Ghadeer Alrefaei,&nbsp;Nouf Alsubhi,&nbsp;Aziza Alrafiah,&nbsp;Safiah Alhazmi\",\"doi\":\"10.2147/BCTT.S408711\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Breast cancer is one of the most common malignancies among women. Recent studies revealed that differentially methylated regions (DMRs) are implicated in regulating gene expression. The goal of this research was to determine which genes and pathways are dysregulated in breast cancer when their promoters are methylated in an abnormal way, leading to differential expression. Whole-genome bisulfite sequencing was applied to analyze DMRs for eight peripheral blood samples collected from five Saudi females diagnosed with stages I and II of breast cancer aligned with three normal females. Three of those patients and three normal samples were used to determine differentially expressed genes (DEG) using Illumina platform NovaSeq PE150.</p><p><strong>Results: </strong>Based on ontology (GO) and KEGG pathways, the analysis indicated that DMGs and DEG are closely related to associated processes, such as ubiquitin-protein transferase activity, ubiquitin-mediated proteolysis, and oxidative phosphorylation. The findings indicated a potentially significant association between global hypomethylation and breast cancer in Saudi patients. Our results revealed 81 differentially promoter-methylated and expressed genes. The most significant differentially methylated and expressed genes found in gene ontology (GO) are pumilio RNA binding family member 1 (<i>PUM1</i>) and zinc finger AN1-type containing 2B (<i>ZFAND2B</i>) also known as (<i>AIRAPL</i>).</p><p><strong>Conclusion: </strong>The essential outcomes of this study suggested that aberrant hypermethylation at crucial genes that have significant parts in the molecular pathways of breast cancer could be used as a potential prognostic biomarker for breast cancer.</p>\",\"PeriodicalId\":9106,\"journal\":{\"name\":\"Breast Cancer : Targets and Therapy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fa/e0/bctt-15-435.PMC10332364.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Breast Cancer : Targets and Therapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/BCTT.S408711\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breast Cancer : Targets and Therapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/BCTT.S408711","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:乳腺癌是女性中最常见的恶性肿瘤之一。最近的研究表明,差异甲基化区(DMRs)参与调控基因表达。这项研究的目的是确定哪些基因和途径在乳腺癌中失调,当它们的启动子以异常的方式甲基化,导致差异表达。采用全基因组亚硫酸氢盐测序分析了5名诊断为乳腺癌I期和II期的沙特女性与3名正常女性的8份外周血样本的DMRs。其中3例患者和3例正常样本使用Illumina平台NovaSeq PE150检测差异表达基因(DEG)。结果:基于本体(GO)和KEGG通路的分析表明,dmg和DEG与泛素蛋白转移酶活性、泛素介导的蛋白水解和氧化磷酸化等相关过程密切相关。研究结果表明,在沙特患者中,整体低甲基化与乳腺癌之间存在潜在的显著关联。我们的研究结果揭示了81个差异启动子甲基化和表达的基因。在基因本体(GO)中发现的差异甲基化和表达最显著的基因是pumilio RNA结合家族成员1 (PUM1)和含锌指an1型2B (ZFAND2B),也称为(AIRAPL)。结论:本研究的主要结果表明,在乳腺癌分子通路中起重要作用的关键基因的异常高甲基化可能被用作乳腺癌的潜在预后生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Promoter Methylation-Regulated Differentially Expressed Genes in Breast Cancer.

Promoter Methylation-Regulated Differentially Expressed Genes in Breast Cancer.

Promoter Methylation-Regulated Differentially Expressed Genes in Breast Cancer.

Promoter Methylation-Regulated Differentially Expressed Genes in Breast Cancer.

Background: Breast cancer is one of the most common malignancies among women. Recent studies revealed that differentially methylated regions (DMRs) are implicated in regulating gene expression. The goal of this research was to determine which genes and pathways are dysregulated in breast cancer when their promoters are methylated in an abnormal way, leading to differential expression. Whole-genome bisulfite sequencing was applied to analyze DMRs for eight peripheral blood samples collected from five Saudi females diagnosed with stages I and II of breast cancer aligned with three normal females. Three of those patients and three normal samples were used to determine differentially expressed genes (DEG) using Illumina platform NovaSeq PE150.

Results: Based on ontology (GO) and KEGG pathways, the analysis indicated that DMGs and DEG are closely related to associated processes, such as ubiquitin-protein transferase activity, ubiquitin-mediated proteolysis, and oxidative phosphorylation. The findings indicated a potentially significant association between global hypomethylation and breast cancer in Saudi patients. Our results revealed 81 differentially promoter-methylated and expressed genes. The most significant differentially methylated and expressed genes found in gene ontology (GO) are pumilio RNA binding family member 1 (PUM1) and zinc finger AN1-type containing 2B (ZFAND2B) also known as (AIRAPL).

Conclusion: The essential outcomes of this study suggested that aberrant hypermethylation at crucial genes that have significant parts in the molecular pathways of breast cancer could be used as a potential prognostic biomarker for breast cancer.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.10
自引率
0.00%
发文量
40
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信