束状猕猴肾细胞培养作为候选疫苗开发和体外模型

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Medical Primatology Pub Date : 2023-06-09 DOI:10.1111/jmp.12655

Rahmat Yusni, Silmi Mariya, Uus Saepuloh, Sela S. Mariya, Huda S. Darusman

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引用次数: 0

摘要

背景细胞培养是指从原始组织中分离或从现有组织中生长的细胞群体在体外增殖。一个重要的来源是猴子肾细胞培养物，它在生物医学研究中发挥着重要作用。这是由于人类和猕猴基因组之间的显著同源性，使得这些基因组可用于培养人类病毒，特别是肠道病毒和培育疫苗。方法本研究从猕猴（Mf）肾脏中提取细胞培养物，并对其基因表达进行验证。结果原代培养成功传代6代，单层生长，呈现上皮样形态。培养的细胞在表型上仍然是异质性的，它们表达CD155和CD46作为病毒受体、细胞形态（CD24、内皮素和vWF）、增殖以及凋亡标记物（Ki67和p53）。结论该细胞培养物可作为疫苗开发和生物活性化合物的体外模型细胞。

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Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Background

Cell culture is the proliferation of a cell population in vitro by isolating from the original tissue or growing from existing ones. One essential source is the monkey kidney cell cultures which have an essential role in biomedical study. This is due to the significant homology between the human and macaque genomes making these useful for cultivating human viruses, especially enteroviruses, and growing vaccines.

Methods

This study developed cell cultures derived from the kidney of Macaca fascicularis (Mf) and validated its gene expression.

Results

The primary cultures were successfully subcultured up to six passages, grew as monolayers, and exhibited epithelial-like morphology. The cultured cells remained heterogeneous in phenotype and they expressed CD155 and CD46 as viral receptors, cell morphology (CD24, endosialin, and vWF), proliferation, also apoptosis markers (Ki67 and p53).

Conclusions

These results indicated that the cell cultures can be used as in vitro model cells for vaccine development and bioactive compound.

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.