电针通过调节小鼠肠道微生物群和增加丁酸生成来促进肠道运动并缓解功能性便秘

IF 4.2 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Ming-min Xu , Yu Guo , Ying Chen , Wei Zhang , Lu Wang , Ying Li
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引用次数: 2

摘要

目的肠道微生物群和肠道短链脂肪酸(SCFA)水平的异常与功能性便秘(FC)的发病机制有关。电针(EA)已被证明可以改善便秘相关症状并重新平衡肠道微生物群。然而,目前尚不清楚肠道微生物群是否是EA的关键机制靶点,也不知道EA如何通过调节肠道微生物群和SCFA来促进肠道运动。因此,我们评估了电针对FC小鼠和伪无菌(PGF)小鼠的影响,以解决这些问题。方法雌性昆明小鼠40只,随机分为正常对照组(n=8)、FC组(n=9)、FC+EA组(n=10)、PGF组(n=12)和PGF+EA组。FC组和FC+EA组用二苯氧基酯治疗建立FC模型;给予PGF组和PGF+EA组抗生素混合物以启动PGF模型。模型维持14d后,FC+EA和PGF+EA组小鼠在ST25和ST37穴位接受电针刺激,每天1次,每周5次,持续2周。计算排便参数和肠道传输率,以评估电针对便秘和胃肠动力的疗效。结肠内容物使用16S rRNA测序来量化肠道微生物多样性,并使用气相色谱-质谱法测量SCFA浓度。结果与FC组相比,电针组在8h内显著缩短了首次黑便排便时间(P<;0.05),并增加了肠道转运率(P<!0.01)、粪粒数(P>;0.05)、湿重(P<?0.05)和含水量(P>!0.01),表明电针组能促进肠道运动,缓解便秘。然而,电针治疗并没有逆转PGF小鼠的慢传输结肠运动(P>;0.05),这表明肠道微生物群可能在电针治疗便秘中发挥机制作用。此外,电针治疗恢复了厚壁菌门与拟杆菌门的比例,并显著增加了FC小鼠的丁酸生成(P<;0.05),这很可能是由于葡萄球菌科微生物的上调(P<!0.01)。结论电针介导的便秘解决是通过重新平衡肠道微生物群和促进丁酸生成来实现的。请引用这篇文章:Xu MM,Guo Y,Chen Y,Zhang W,Wang L,Li Y。电针通过调节小鼠肠道微生物群和增加丁酸的产生,促进肠道运动,缓解功能性便秘。国际医学杂志,2023;Epub在印刷之前。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electro-acupuncture promotes gut motility and alleviates functional constipation by regulating gut microbiota and increasing butyric acid generation in mice

Objective

Abnormalities in the gut microbiota and intestinal short-chain fatty acid (SCFA) levels are implicated in the pathogenesis of functional constipation (FC). Electro-acupuncture (EA) has been shown to improve constipation-related symptoms and rebalance the gut microbiota. However, it is currently unknown whether the gut microbiota is a key mechanistic target for EA or how EA promotes gut motility by regulating the gut microbiota and SCFAs. Therefore, we assessed the effects of EA in FC mice and pseudo-germfree (PGF) mice to address these questions.

Methods

Forty female Kunming mice were randomly separated into a normal control group (n = 8), an FC group (n = 8), an FC + EA group (n = 8), a PGF group (n = 8) and a PGF + EA group (n = 8). The FC group and FC + EA group were treated with diphenoxylate to establish the FC model; the PGF group and PGF + EA group were given an antibiotic cocktail to initiate the PGF model. After maintaining the model for 14 d, mice in the FC + EA and PGF + EA groups received EA stimulation at the ST25 and ST37 acupoints, once a day, 5 times per week, for 2 weeks. Fecal parameters and intestinal transit rate were calculated to assess the efficacy of EA on constipation and gastrointestinal motility. Colonic contents were used to quantify gut microbial diversity using 16S rRNA sequencing, and measure SCFA concentrations using gas chromatography-mass spectrometry.

Results

EA significantly shortened the first black stool defecation time (P < 0.05) and increased the intestinal transit rate (P < 0.01), and fecal pellet number (P < 0.05), wet weight (P < 0.05) and water content (P < 0.01) over 8 h, compared with the FC group, showing that EA promoted gut motility and alleviated constipation. However, EA treatment did not reverse slow-transit colonic motility in PGF mice (P > 0.05), demonstrating that the gut microbiota may play a mechanistic role in the EA treatment of constipation. In addition, EA treatment restored the Firmicutes to Bacteroidetes ratio and significantly increased butyric acid generation in FC mice (P < 0.05), most likely due to the upregulation of Staphylococcaceae microorganisms (P < 0.01).

Conclusion

EA-mediated resolution of constipation occurs through rebalancing the gut microbiota and promoting butyric acid generation.

Please cite this article as: Xu MM, Guo Y, Chen Y, Zhang W, Wang L, Li Y. Electro-acupuncture promotes gut motility and alleviates functional constipation by regulating gut microbiota and increasing butyric acid generation in mice. J Integr Med. 2023; Epub ahead of print.

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来源期刊
Journal of Integrative Medicine-Jim
Journal of Integrative Medicine-Jim Medicine-Complementary and Alternative Medicine
CiteScore
9.20
自引率
4.20%
发文量
3319
期刊介绍: The predecessor of JIM is the Journal of Chinese Integrative Medicine (Zhong Xi Yi Jie He Xue Bao). With this new, English-language publication, we are committed to make JIM an international platform for publishing high-quality papers on complementary and alternative medicine (CAM) and an open forum in which the different professions and international scholarly communities can exchange views, share research and their clinical experience, discuss CAM education, and confer about issues and problems in our various disciplines and in CAM as a whole in order to promote integrative medicine. JIM is indexed/abstracted in: MEDLINE/PubMed, ScienceDirect, Emerging Sources Citation Index (ESCI), Scopus, Embase, Chemical Abstracts (CA), CAB Abstracts, EBSCO, WPRIM, JST China, Chinese Science Citation Database (CSCD), and China National Knowledge Infrastructure (CNKI). JIM Editorial Office uses ThomsonReuters ScholarOne Manuscripts as submitting and review system (submission link: http://mc03.manuscriptcentral.com/jcim-en). JIM is published bimonthly. Manuscripts submitted to JIM should be written in English. Article types include but are not limited to randomized controlled and pragmatic trials, translational and patient-centered effectiveness outcome studies, case series and reports, clinical trial protocols, preclinical and basic science studies, systematic reviews and meta-analyses, papers on methodology and CAM history or education, conference proceedings, editorials, commentaries, short communications, book reviews, and letters to the editor. Our purpose is to publish a prestigious international journal for studies in integrative medicine. To achieve this aim, we seek to publish high-quality papers on any aspects of integrative medicine, such as acupuncture and traditional Chinese medicine, Ayurveda medicine, herbal medicine, homeopathy, nutrition, chiropractic, mind-body medicine, taichi, qigong, meditation, and any other modalities of CAM; our commitment to international scope ensures that research and progress from all regions of the world are widely covered. These ensure that articles published in JIM have the maximum exposure to the international scholarly community. JIM can help its authors let their papers reach the widest possible range of readers, and let all those who share an interest in their research field be concerned with their study.
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