阿尔及利亚肺结核的综合耐药特征:结核分枝杆菌菌株全基因组测序的见解。

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES

Microbial drug resistance Pub Date : 2023-07-01 DOI:10.1089/mdr.2022.0321

Dalila Benremila, Ferhat Djoudi, Alima Gharout-Sait, Slimane Kheloufi, Andrea Spitaleri, Simone Battaglia, Andrea Maurizio Cabibbe, Daniela Maria Cirillo

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引用次数: 0

摘要

在本研究中，我们旨在通过全基因组测序(WGS)来表征耐药菌株，描述其传播谱系和传播历史。采用96孔肉汤微量稀释板进行药敏试验。提取并纯化基因组DNA;在Illumina nextq500系统上制备和运行文库。在82株结核(TB)分离株中，21株(25.6%)耐药，其中耐多药10株，广泛耐药(XDR)结核4株。在15株分离株中证实了katG基因Ser315Thr突变。在rpoB中，Ser450Leu和His445Asp突变最为常见。在gyrA中报道了Asp94Asn和Ala90Val突变。LAM家族是最具结核病耐药性的家族，在该国北部和南部的T亚系广泛占主导地位。这项研究首次利用WGS了解了阿尔及利亚的结核病耐药性，并清楚地描述了该国首批广泛耐药前结核病病例和谱系分布。

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Comprehensive Drug Resistance Characterization of Pulmonary Tuberculosis in Algeria: Insights on Mycobacterium tuberculosis Strains by Whole-Genome Sequencing.

In this study, we aimed to characterize drug-resistant strains by whole-genome sequencing (WGS), to describe the spreading lineages and the history of transmission. Drug susceptibility testing was performed by 96-well broth microdilution plates. The genomic DNA was extracted and purified; libraries were prepared and run on the Illumina NextSeq500 System. Among 82 isolates, 21 tuberculosis (TB) isolates (25.6%) were drug resistant, including 10 MDR and 4 pre-extensively drug-resistant (XDR)-TB. The mutation Ser315Thr in the katG gene was confirmed in 15 isolates. In rpoB, Ser450Leu and His445Asp mutations were the most common. Asp94Asn and Ala90Val mutations were reported in gyrA. The LAM family, the most TB drug resistant, was widely predominant in the north and the T sublineage in the south of the country. This study provides the first insight on TB drug resistance using WGS in Algeria and clearly describes the first pre-XDR-TB cases and lineage distribution across the country.

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.