肠沙门氏菌血清型鼠伤寒杆菌的双精氨酸易位突变体对细胞壁靶向抗生素的敏感性增加。

Adrienne M Brauer, Alexandra R Rogers, Jeremy R Ellermeier
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引用次数: 2

摘要

双精氨酸易位(Tat)系统是一种在细菌、古生菌和植物中保守存在的蛋白质分泌系统。在革兰氏阴性菌中,它是将折叠蛋白从细胞质输出到外周质所必需的。沙门氏菌中有30种实验证实的Tat底物,包括氢化酶亚基、厌氧呼吸所需的酶和细胞分裂过程中参与肽聚糖重塑的酶。多项研究表明,该突变体对SDS和胆汁等抗菌化合物敏感;然而,在这项工作中,我们使用生长曲线和活菌板计数来证明细胞壁靶向抗生素(青霉素类、碳青霉烯类、头孢菌素和磷霉素)增加了对Δtat菌株的杀伤。此外,我们证明这种增加的杀伤主要是由于关键Tat底物的易位缺陷:MepK, AmiA, AmiC和SufI。最后,我们表明ΔhyaAB ΔhybABC ΔhydBC菌株具有改变的ΔΨ,影响有氧生长条件下关键Tat底物的正常分泌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Twin-arginine translocation (Tat) mutants in <i>Salmonella enterica</i> serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics.

Twin-arginine translocation (Tat) mutants in <i>Salmonella enterica</i> serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics.

Twin-arginine translocation (Tat) mutants in <i>Salmonella enterica</i> serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics.

Twin-arginine translocation (Tat) mutants in Salmonella enterica serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics.

The twin-arginine translocation (Tat) system is a protein secretion system that is conserved in bacteria, archaea and plants. In Gram-negative bacteria, it is required for the export of folded proteins from the cytoplasm to the periplasm. There are 30 experimentally verified Tat substrates in Salmonella, including hydrogenase subunits, enzymes required for anaerobic respiration and enzymes involved in peptidoglycan remodeling during cell division. Multiple studies have demonstrated the susceptibility of tat mutants to antimicrobial compounds such as SDS and bile; however, in this work, we use growth curves and viable plate counts to demonstrate that cell wall targeting antibiotics (penicillins, carbapenems, cephalosporins and fosfomycin) have increased killing against a Δtat strain. Further, we demonstrate that this increased killing is primarily due to defects in translocation of critical Tat substrates: MepK, AmiA, AmiC and SufI. Finally, we show that a ΔhyaAB ΔhybABC ΔhydBC strain has an altered ΔΨ that impacts proper secretion of critical Tat substrates in aerobic growth conditions.

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CiteScore
3.30
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