{"title":"环境分离物过氧化氢酶基因的自克隆提高了其在琼脂培养基上的集落形成能力。","authors":"Motoyuki Watanabe, Kensuke Igarashi, Souichiro Kato, Yoichi Kamagata, Wataru Kitagawa","doi":"10.1264/jsme2.ME23006","DOIUrl":null,"url":null,"abstract":"<p><p>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H<sub>2</sub>O<sub>2</sub> concentration in agar plates. Putative catalase genes, which degrade H<sub>2</sub>O<sub>2</sub>, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H<sub>2</sub>O<sub>2</sub> pressure. The present results demonstrated high sensitivity to H<sub>2</sub>O<sub>2</sub> even in microbes possessing functional catalase genes.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 2","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308234/pdf/","citationCount":"0","resultStr":"{\"title\":\"Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media.\",\"authors\":\"Motoyuki Watanabe, Kensuke Igarashi, Souichiro Kato, Yoichi Kamagata, Wataru Kitagawa\",\"doi\":\"10.1264/jsme2.ME23006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H<sub>2</sub>O<sub>2</sub> concentration in agar plates. Putative catalase genes, which degrade H<sub>2</sub>O<sub>2</sub>, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H<sub>2</sub>O<sub>2</sub> pressure. The present results demonstrated high sensitivity to H<sub>2</sub>O<sub>2</sub> even in microbes possessing functional catalase genes.</p>\",\"PeriodicalId\":18482,\"journal\":{\"name\":\"Microbes and Environments\",\"volume\":\"38 2\",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308234/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbes and Environments\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1264/jsme2.ME23006\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbes and Environments","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1264/jsme2.ME23006","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media.
Hydrogen peroxide (H2O2) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H2O2 concentration in agar plates. Putative catalase genes, which degrade H2O2, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H2O2 pressure. The present results demonstrated high sensitivity to H2O2 even in microbes possessing functional catalase genes.
期刊介绍：
Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.
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