{"title":"软性隐形眼镜使用者和隐形眼镜不适患者的结膜微生物组变化。","authors":"Kang Xiao, Hang Song, Zhengyu Chen, Qin Long","doi":"10.1080/1354750X.2023.2229532","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Contact lens discomfort (CLD) acts as a challenging problem, and the associated conjunctival microbiome changes were unclear.</p><p><strong>Material and methods: </strong>Conjunctival sac swab samples were collected from 12 eyes of nonwearers (NW), 12 eyes of asymptomatic contact lens (ACL) wearers, and 11 eyes of CLD. The V3-V4 region of the 16S rRNA gene sequencing was used to investigate differences among three groups.</p><p><strong>Results: </strong>No differences in alpha diversity were observed among the three groups. The beta diversity showed a distinct microbiome composition between ACL and CLD group (<i>P</i> = 0.018) with principal coordinate analysis. The relative abundance of <i>Firmicutes</i> was significantly higher in CLD (48.18%) than in ACL (13.21%) group (<i>P</i> = 0.018). The abundance of <i>Bacillus</i> in patients with ACL (0.05%) or with CLD (0.02%) were significantly lower than that in the NW (1.27%) group (<i>P</i> = 0.024, 0.028, respectively). Moreover, the abundance of <i>Firmicutes</i> was positively correlated with the OSDI scores in CLD patients (r = 0.817, <i>P</i> < 0. 01, Spearman).</p><p><strong>Discussions: </strong>Patients with CLD have various degrees of bacterial microbiota imbalance in the conjunctival sac, compared with NW and ACL groups.</p><p><strong>Conclusion: </strong><i>Firmicutes</i> may serve as a potential biomarker for the CLD patients.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"531-537"},"PeriodicalIF":2.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Conjunctival microbiome changes in soft contact lens users and contact lens discomfort patients.\",\"authors\":\"Kang Xiao, Hang Song, Zhengyu Chen, Qin Long\",\"doi\":\"10.1080/1354750X.2023.2229532\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Contact lens discomfort (CLD) acts as a challenging problem, and the associated conjunctival microbiome changes were unclear.</p><p><strong>Material and methods: </strong>Conjunctival sac swab samples were collected from 12 eyes of nonwearers (NW), 12 eyes of asymptomatic contact lens (ACL) wearers, and 11 eyes of CLD. The V3-V4 region of the 16S rRNA gene sequencing was used to investigate differences among three groups.</p><p><strong>Results: </strong>No differences in alpha diversity were observed among the three groups. The beta diversity showed a distinct microbiome composition between ACL and CLD group (<i>P</i> = 0.018) with principal coordinate analysis. The relative abundance of <i>Firmicutes</i> was significantly higher in CLD (48.18%) than in ACL (13.21%) group (<i>P</i> = 0.018). The abundance of <i>Bacillus</i> in patients with ACL (0.05%) or with CLD (0.02%) were significantly lower than that in the NW (1.27%) group (<i>P</i> = 0.024, 0.028, respectively). Moreover, the abundance of <i>Firmicutes</i> was positively correlated with the OSDI scores in CLD patients (r = 0.817, <i>P</i> < 0. 01, Spearman).</p><p><strong>Discussions: </strong>Patients with CLD have various degrees of bacterial microbiota imbalance in the conjunctival sac, compared with NW and ACL groups.</p><p><strong>Conclusion: </strong><i>Firmicutes</i> may serve as a potential biomarker for the CLD patients.</p>\",\"PeriodicalId\":8921,\"journal\":{\"name\":\"Biomarkers\",\"volume\":\" \",\"pages\":\"531-537\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomarkers\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/1354750X.2023.2229532\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomarkers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/1354750X.2023.2229532","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/11 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Conjunctival microbiome changes in soft contact lens users and contact lens discomfort patients.
Introduction: Contact lens discomfort (CLD) acts as a challenging problem, and the associated conjunctival microbiome changes were unclear.
Material and methods: Conjunctival sac swab samples were collected from 12 eyes of nonwearers (NW), 12 eyes of asymptomatic contact lens (ACL) wearers, and 11 eyes of CLD. The V3-V4 region of the 16S rRNA gene sequencing was used to investigate differences among three groups.
Results: No differences in alpha diversity were observed among the three groups. The beta diversity showed a distinct microbiome composition between ACL and CLD group (P = 0.018) with principal coordinate analysis. The relative abundance of Firmicutes was significantly higher in CLD (48.18%) than in ACL (13.21%) group (P = 0.018). The abundance of Bacillus in patients with ACL (0.05%) or with CLD (0.02%) were significantly lower than that in the NW (1.27%) group (P = 0.024, 0.028, respectively). Moreover, the abundance of Firmicutes was positively correlated with the OSDI scores in CLD patients (r = 0.817, P < 0. 01, Spearman).
Discussions: Patients with CLD have various degrees of bacterial microbiota imbalance in the conjunctival sac, compared with NW and ACL groups.
Conclusion: Firmicutes may serve as a potential biomarker for the CLD patients.
期刊介绍：
The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source.
Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged:
• Biomarkers of disease
• Biomarkers of exposure
• Biomarkers of response
• Biomarkers of susceptibility
Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.
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