描述难以培养的细菌:走捷径还是花时间发现新东西?

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Svetlana N. Dedysh
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引用次数: 0

摘要

尽管人们对分离研究不足和尚未培养的细菌系统发育群的代表越来越感兴趣,但这些微生物仍然是分类学研究的难点。描述这些挑剔细菌所需的时间通常以几年为单位。更成问题的是,许多最初为快速生长和快速反应的微生物开发的常规实验室测试并不完全适合许多与环境相关的缓慢生长的细菌。化学组学分析中使用的标准技术无法识别这些细菌产生的独特脂质。一种常见的做法是准备分类描述,报告一组最小的特征来命名新分离的生物体,这加深了微生物生态学家和分类学家之间的差距。相比之下,投入时间对细胞生物学进行详细分析,并对新分离的微生物的基因组编码能力进行实验验证,为新的、意想不到的发现打开了一扇窗户,这可能会影响我们对这些微生物在环境中的功能作用的看法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Describing difficult-to-culture bacteria: Taking a shortcut or investing time to discover something new?

Despite the growing interest in isolating representatives of poorly studied and as-yet-uncultivated bacterial phylogenetic groups, these microorganisms remain difficult objects for taxonomic studies. The time required for describing one of these fastidious bacteria is commonly measured in several years. What is even more problematic, many routine laboratory tests, which were originally developed for fast-growing and fast-responding microorganisms, are not fully suitable for many environmentally relevant, slow-growing bacteria. Standard techniques used in chemotaxonomic analyses do not identify unique lipids produced by these bacteria. A common practice of preparing taxonomic descriptions that report a minimal set of features to name a newly isolated organism deepens a gap between microbial ecologists and taxonomists. By contrast, investing time in detailed analysis of cell biology and experimental verification of genome-encoded capabilities of newly isolated microorganisms opens a window for novel, unexpected findings, which may shape our ideas about the functional role of these microbes in the environment.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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