精氨酸加压素对大鼠下丘脑视前区温度敏感和温度不敏感神经元膜电位的影响

IF 2.5 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Yu Tang , Siyuan Liu , Lingzhi Xu , Min Huang , Ke Zhang
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引用次数: 0

摘要

精氨酸加压素(AVP)在体温调节中起低温调节作用,是该机制中重要的内源性介质。在视前区(POA),AVP增加了温敏神经元的自发放电和热敏性,降低了冷敏和温度不敏感神经元的自发发射和热敏性。由于POA神经元在精确的体温调节反应中起着至关重要的作用,这些发现表明低温与AVP诱导的POA神经元的放电活性变化之间存在关联。然而,AVP控制这种放电活动的电生理机制尚不清楚。因此,在本研究中,使用体外下丘脑脑切片和全细胞记录,我们阐明了温度敏感和不敏感的POA神经元的膜电位反应,以确定AVP或V1a加压素受体拮抗剂的应用。通过监测实验灌注前和灌注过程中神经元静息电位和膜电位热敏性的变化,我们观察到AVP增加了50%的温度不敏感神经元静息电位的变化,但减少了其他神经元的静息电位变化。这些变化是因为AVP增强了近50%的温度不敏感神经元的膜电位热敏性。另一方面,AVP改变了温度敏感神经元的静息电位和膜电位热敏性,而温敏神经元和冷敏神经元之间没有差异。在AVP或V1a加压素受体拮抗剂灌注之前和灌注期间,未观察到所有神经元的热敏性和膜电位变化之间的相关性。此外,在实验灌注过程中,神经元的热敏性和膜电位热敏性之间没有观察到相关性。在本研究中,我们发现AVP诱导没有导致静息电位的任何变化,这是温度敏感神经元所特有的。研究结果表明,AVP引起的POA神经元放电活性和放电速率热敏性的变化不受静息电位的控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Arginine vasopressin effects on membrane potentials of preoptic area temperature-sensitive and -insensitive neurons in rat hypothalamic tissue slices

Arginine vasopressin (AVP) plays a hypothermic regulatory role in thermoregulation and is an important endogenous mediator in this mechanism. In the preoptic area (POA), AVP increases the spontaneous firing and thermosensitivity of warm-sensitive neurons and decreases those of cold-sensitive and temperature-insensitive neurons. Because POA neurons play a crucial role in precise thermoregulatory responses, these findings indicate that there is an association between the hypothermia and changes in the firing activity of AVP-induced POA neurons. However, the electrophysiological mechanisms by which AVP controls this firing activity remain unclear. Therefore, in the present study, using in vitro hypothalamic brain slices and whole-cell recordings, we elucidated the membrane potential responses of temperature-sensitive and –insensitive POA neurons to identify the applications of AVP or V1a vasopressin receptor antagonists. By monitoring changes in the resting potential and membrane potential thermosensitivity of the neurons before and during experimental perfusion, we observed that AVP increased the changes in the resting potential of 50% of temperature-insensitive neurons but reduced them in others. These changes are because AVP enhances the membrane potential thermosensitivity of nearly 50% of the temperature-insensitive neurons. On the other hand, AVP changes both the resting potential and membrane potential thermosensitivity of temperature-sensitive neurons, with no differences between the warm- and cold-sensitive neurons. Before and during AVP or V1a vasopressin receptor antagonist perfusion, no correlation was observed between changes in the thermosensitivity and membrane potential of all neurons. Furthermore, no correlation was observed between the thermosensitivity and membrane potential thermosensitivity of the neurons during experimental perfusion. In the present study, we found that AVP induction did not result in any changes in resting potential, which is unique to temperature-sensitive neurons. The study results suggest that AVP-induced changes in the firing activity and firing rate thermosensitivity of POA neurons are not controlled by resting potentials.

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来源期刊
Neuropeptides
Neuropeptides 医学-内分泌学与代谢
CiteScore
5.40
自引率
6.90%
发文量
55
审稿时长
>12 weeks
期刊介绍: The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.
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