在细菌感染和抗生素治疗后,黄颡鱼幼鱼的皮肤、鳃和肠道黏膜微生物群的破坏。

Daniela Rosado, Paula Canada, Sofia Marques Silva, Nuno Ribeiro, Pedro Diniz, Raquel Xavier
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引用次数: 0

摘要

鱼类粘膜微生物群的活性提供了与免疫反应、消化或代谢有关的功能。一些生物和非生物因素有助于维持微生物稳态,破坏导致生态失调。众所周知,疾病和抗生素的使用会导致养殖鱼类的生态失调。病原菌感染极大地影响了鳙鱼的生产,仍然经常需要抗生素治疗。在这里,我们采用16S rRNA高通量元组学方法来表征由于感染光杆菌damselae亚sp而发生的肠道、皮肤和鳃微生物组的变化。随后用土霉素(OTC)进行抗生素治疗,以及在康复期间。尽管所研究的组织中微生物群的反应不同,但在所有粘膜中都观察到组成、多样性、结构和预测功能的总体变化。患病鱼类的皮肤和鳃微生物群主要由经常与继发性感染相关的分类群控制,而在肠道中,已知包括致病菌的弧菌属随着OTC治疗而增加。该研究强调了疾病和抗生素治疗对养殖鱼类微生物群的负面影响。我们的研究结果还表明,鱼类运输操作可能对鱼类微生物群产生深远的影响,但需要进一步的研究来准确评估其影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Disruption of the skin, gill, and gut mucosae microbiome of gilthead seabream fingerlings after bacterial infection and antibiotic treatment.

Disruption of the skin, gill, and gut mucosae microbiome of gilthead seabream fingerlings after bacterial infection and antibiotic treatment.

Disruption of the skin, gill, and gut mucosae microbiome of gilthead seabream fingerlings after bacterial infection and antibiotic treatment.

Disruption of the skin, gill, and gut mucosae microbiome of gilthead seabream fingerlings after bacterial infection and antibiotic treatment.

The activity of the microbiome of fish mucosae provides functions related to immune response, digestion, or metabolism. Several biotic and abiotic factors help maintaining microbial homeostasis, with disruptions leading to dysbiosis. Diseases and antibiotic administration are known to cause dysbiosis in farmed fish. Pathogen infections greatly affect the production of gilthead seabream, and antibiotic treatment is still frequently required. Here, we employed a 16S rRNA high-throughput metataxonomics approach to characterize changes in the gut, skin, and gill microbiomes occurring due to infection with Photobacterium damselae subsp. piscicida and subsequent antibiotic treatment with oxytetracycline (OTC), as well as during recovery. Although microbiota response differed between studied tissues, overall changes in composition, diversity, structure, and predicted function were observed in all mucosae. The skin and gill microbiomes of diseased fish became largely dominated by taxa that have been frequently linked to secondary infections, whereas in the gut the genus Vibrio, known to include pathogenic bacteria, increased with OTC treatment. The study highlights the negative impacts of disease and antibiotic treatment on the microbiome of farmed fish. Our results also suggest that fish transportation operations may have profound effects on the fish microbiome, but further studies are needed to accurately evaluate their impact.

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