甘油磷酸乙醇胺在肥胖相关哮喘中的分子种类。

Q3 Biochemistry, Genetics and Molecular Biology
Yu K Denisenko, U M Omatova, T P Novgorodtseva, E V Ermolenko
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引用次数: 0

摘要

支气管哮喘(BA)合并肥胖是一种进行性疾病表型,很难对标准治疗作出反应。在这方面,阐明这种共病病理发展的细胞和分子机制是很重要的。近年来,脂质组学已成为一种活跃的研究工具,不仅为了解健康和疾病的细胞过程,而且为提供个性化的医学方法提供了新的机会。本研究的目的是通过研究BA合并肥胖患者血浆中甘油磷脂酰乙醇胺(GPEs)的分子种类来表征脂质组表型。对11例患者血液样本中gpe的分子种类进行了研究。采用高分辨率串联质谱法对gpe进行鉴定和定量。在该病理学中,首次显示了血浆中二酰基、烷基酰基和烷基酰基HPEs分子种类的脂质谱变化。在BA合并肥胖患者中,二酰基磷酸乙醇胺分子组成sn2位点上以酰基18:2和20:4为主。在脂肪酸(FA)比例为20:4、22:4和18:2的GPE二酰基含量增加的同时,这些脂肪酸在GPE的烷基和烯基分子种中有所减少,表明它们在亚类之间重新分布。BA合并肥胖患者烯基GPEs sn2位点的二十碳五烯酸(20:5)缺乏表明合成抗炎介质的底物减少。在醚型分子种类缺乏的情况下,由于二酰基GPE含量的显著增加,导致GPE亚类分布的不平衡,可能引起慢性炎症和氧化应激的发展。在BA合并肥胖的过程中,以GPE分子种的基本组成和化学结构的改变为特征的公认脂质组谱表明它们参与了BA发生的发病机制。阐明单个甘油磷脂亚类及其个体成员的特殊作用可能有助于确定新的治疗靶点和支气管肺病理的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular species of glycerophosphoethanolamines in obesity-associated asthma.

Bronchial asthma (BA) complicated by obesity is a progressive disease phenotype that hardly responds to standard therapy. In this regard, it is important to elucidate cellular and molecular mechanisms of development of this comorbid pathology. In recent years, lipidomics has become an active research tool, opening new opportunities not only for understanding cellular processes in health and disease, but also for providing a personalized approach to medicine. The aim of this study was to characterize the lipidome phenotype based on the study of molecular species of glycerophosphatidylethanolamines (GPEs) in blood plasma of patients with BA complicated by obesity. Molecular species of GPEs were studied in blood samples of 11 patients. Identification and quantification of GPEs was carried out using high resolution tandem mass spectrometry. For the first time in this pathology, a change in the lipidome profile of molecular species of diacyl, alkyl-acyl and alkenyl-acyl HPEs of blood plasma was shown. In BA complicated by obesity, acyl groups 18:2 and 20:4 were dominated in the sn2 position of the molecular composition of diacylphosphoethanolamines. Simultaneously with the increase in the level of GPE diacyls with the fatty acids (FA) 20:4, 22:4, and 18:2, there was a decrease in these FAs in alkyl and alkenyl molecular species of GPEs, thus indicating their redistribution between subclasses. The eicosapentaenoic acid (20:5) deficiency at the sn2 position of alkenyl GPEs in patients with BA complicated by obesity indicates a decrease in the substrate for the synthesis of anti-inflammatory mediators. The resulting imbalance in the distribution of GPE subclasses, due to a pronounced increase in the content of diacyl GPE under conditions of the deficiency of molecular species of ether forms, can probably cause chronic inflammation and the development of oxidative stress. The recognized lipidome profile characterized by the modification of the basic composition and the chemical structure of GPE molecular species in BA complicated by obesity indicates their involvement in the pathogenetic mechanisms underlying BA development. The elucidation of particular roles of individual subclasses of glycerophospholipids and their individual members may contribute to the identification of new therapeutic targets and biomarkers of bronchopulmonary pathology.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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