{"title":"分泌DKK3对肝癌患者循环CD56bright自然杀伤细胞的影响","authors":"Da-Hua Liu, Gui-Min Wen, Chang-Liang Song, Pu Xia","doi":"10.1177/03936155231169796","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Liver cancer seriously threatens human health. Natural killer (NK) cells are an important part of the innate immune system and have strong anti-tumor ability. Immunotherapy based on NK cells has become a hot topic in the treatment of liver cancer.</p><p><strong>Methods: </strong>In this study, we checked the serum DKK3 (sDKK3) and circulating CD56<sup>bright</sup> NK cells using ELISA and flow cytometry, respectively, in the blood of liver cancer patients. The effect on recombinant human DKK3 (rhDKK3) on CD56<sup>bright</sup> NK cells was analyzed in vitro.</p><p><strong>Results: </strong>We found low levels of sDKK3 in liver cancer patients and a negative correlation between sDKK3 and circulating CD56<sup>bright</sup> NK cells. In addition, we found that DKK3 induced the differentiation and improved the cytotoxicity of CD56<sup>bright</sup> NK cells for the first time. It could be used as an agonist for NK cell-based immunotherapy.</p><p><strong>Conclusions: </strong>Improving the clinical efficacy of NK cells through DKK3 will become a new strategy for cancer immunotherapy.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of secretory DKK3 on circulating CD56<sup>bright</sup> natural killer cells in patients with liver cancer.\",\"authors\":\"Da-Hua Liu, Gui-Min Wen, Chang-Liang Song, Pu Xia\",\"doi\":\"10.1177/03936155231169796\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Liver cancer seriously threatens human health. Natural killer (NK) cells are an important part of the innate immune system and have strong anti-tumor ability. Immunotherapy based on NK cells has become a hot topic in the treatment of liver cancer.</p><p><strong>Methods: </strong>In this study, we checked the serum DKK3 (sDKK3) and circulating CD56<sup>bright</sup> NK cells using ELISA and flow cytometry, respectively, in the blood of liver cancer patients. The effect on recombinant human DKK3 (rhDKK3) on CD56<sup>bright</sup> NK cells was analyzed in vitro.</p><p><strong>Results: </strong>We found low levels of sDKK3 in liver cancer patients and a negative correlation between sDKK3 and circulating CD56<sup>bright</sup> NK cells. In addition, we found that DKK3 induced the differentiation and improved the cytotoxicity of CD56<sup>bright</sup> NK cells for the first time. It could be used as an agonist for NK cell-based immunotherapy.</p><p><strong>Conclusions: </strong>Improving the clinical efficacy of NK cells through DKK3 will become a new strategy for cancer immunotherapy.</p>\",\"PeriodicalId\":50334,\"journal\":{\"name\":\"International Journal of Biological Markers\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological Markers\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/03936155231169796\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Markers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/03936155231169796","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Effect of secretory DKK3 on circulating CD56bright natural killer cells in patients with liver cancer.
Background: Liver cancer seriously threatens human health. Natural killer (NK) cells are an important part of the innate immune system and have strong anti-tumor ability. Immunotherapy based on NK cells has become a hot topic in the treatment of liver cancer.
Methods: In this study, we checked the serum DKK3 (sDKK3) and circulating CD56bright NK cells using ELISA and flow cytometry, respectively, in the blood of liver cancer patients. The effect on recombinant human DKK3 (rhDKK3) on CD56bright NK cells was analyzed in vitro.
Results: We found low levels of sDKK3 in liver cancer patients and a negative correlation between sDKK3 and circulating CD56bright NK cells. In addition, we found that DKK3 induced the differentiation and improved the cytotoxicity of CD56bright NK cells for the first time. It could be used as an agonist for NK cell-based immunotherapy.
Conclusions: Improving the clinical efficacy of NK cells through DKK3 will become a new strategy for cancer immunotherapy.
期刊介绍：
IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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