Jeong Moo Han, Ha-Yeon Song, Jong-Hyun Jung, Sangyong Lim, Ho Seong Seo, Woo Sik Kim, Seung-Taik Lim, Eui-Baek Byun
{"title":"耐辐射球菌衍生的膜囊泡通过调节MAPK和Nrf2/ARE途径保护HaCaT细胞免受h2o2诱导的氧化应激。","authors":"Jeong Moo Han, Ha-Yeon Song, Jong-Hyun Jung, Sangyong Lim, Ho Seong Seo, Woo Sik Kim, Seung-Taik Lim, Eui-Baek Byun","doi":"10.1186/s12575-023-00211-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Deinococcus radiodurans is a robust bacterium that can withstand harsh environments that cause oxidative stress to macromolecules due to its cellular structure and physiological functions. Cells release extracellular vesicles for intercellular communication and the transfer of biological information; their payload reflects the status of the source cells. Yet, the biological role and mechanism of Deinococcus radiodurans-derived extracellular vesicles remain unclear.</p><p><strong>Aim: </strong>This study investigated the protective effects of membrane vesicles derived from D. radiodurans (R1-MVs) against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT cells.</p><p><strong>Results: </strong>R1-MVs were identified as 322 nm spherical molecules. Pretreatment with R1-MVs inhibited H<sub>2</sub>O<sub>2</sub>-mediated apoptosis in HaCaT cells by suppressing the loss of mitochondrial membrane potential and reactive oxygen species (ROS) production. R1-MVs increased the superoxide dismutase (SOD) and catalase (CAT) activities, restored glutathione (GSH) homeostasis, and reduced malondialdehyde (MDA) production in H<sub>2</sub>O<sub>2</sub>-exposed HaCaT cells. Moreover, the protective effect of R1-MVs against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT cells was dependent on the downregulation of mitogen-activated protein kinase (MAPK) phosphorylation and the upregulation of the nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Furthermore, the weaker protective capabilities of R1-MVs derived from ΔDR2577 mutant than that of the wild-type R1-MVs confirmed our inferences and indicated that SlpA protein plays a crucial role in R1-MVs against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress.</p><p><strong>Conclusion: </strong>Taken together, R1-MVs exert significant protective effects against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in keratinocytes and have the potential to be applied in radiation-induced oxidative stress models.</p>","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":"25 1","pages":"17"},"PeriodicalIF":3.7000,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10273539/pdf/","citationCount":"2","resultStr":"{\"title\":\"Deinococcus radiodurans-derived membrane vesicles protect HaCaT cells against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress via modulation of MAPK and Nrf2/ARE pathways.\",\"authors\":\"Jeong Moo Han, Ha-Yeon Song, Jong-Hyun Jung, Sangyong Lim, Ho Seong Seo, Woo Sik Kim, Seung-Taik Lim, Eui-Baek Byun\",\"doi\":\"10.1186/s12575-023-00211-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Deinococcus radiodurans is a robust bacterium that can withstand harsh environments that cause oxidative stress to macromolecules due to its cellular structure and physiological functions. Cells release extracellular vesicles for intercellular communication and the transfer of biological information; their payload reflects the status of the source cells. Yet, the biological role and mechanism of Deinococcus radiodurans-derived extracellular vesicles remain unclear.</p><p><strong>Aim: </strong>This study investigated the protective effects of membrane vesicles derived from D. radiodurans (R1-MVs) against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT cells.</p><p><strong>Results: </strong>R1-MVs were identified as 322 nm spherical molecules. Pretreatment with R1-MVs inhibited H<sub>2</sub>O<sub>2</sub>-mediated apoptosis in HaCaT cells by suppressing the loss of mitochondrial membrane potential and reactive oxygen species (ROS) production. R1-MVs increased the superoxide dismutase (SOD) and catalase (CAT) activities, restored glutathione (GSH) homeostasis, and reduced malondialdehyde (MDA) production in H<sub>2</sub>O<sub>2</sub>-exposed HaCaT cells. Moreover, the protective effect of R1-MVs against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT cells was dependent on the downregulation of mitogen-activated protein kinase (MAPK) phosphorylation and the upregulation of the nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Furthermore, the weaker protective capabilities of R1-MVs derived from ΔDR2577 mutant than that of the wild-type R1-MVs confirmed our inferences and indicated that SlpA protein plays a crucial role in R1-MVs against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress.</p><p><strong>Conclusion: </strong>Taken together, R1-MVs exert significant protective effects against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in keratinocytes and have the potential to be applied in radiation-induced oxidative stress models.</p>\",\"PeriodicalId\":8960,\"journal\":{\"name\":\"Biological Procedures Online\",\"volume\":\"25 1\",\"pages\":\"17\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2023-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10273539/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biological Procedures Online\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s12575-023-00211-4\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Procedures Online","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12575-023-00211-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Deinococcus radiodurans-derived membrane vesicles protect HaCaT cells against H2O2-induced oxidative stress via modulation of MAPK and Nrf2/ARE pathways.
Background: Deinococcus radiodurans is a robust bacterium that can withstand harsh environments that cause oxidative stress to macromolecules due to its cellular structure and physiological functions. Cells release extracellular vesicles for intercellular communication and the transfer of biological information; their payload reflects the status of the source cells. Yet, the biological role and mechanism of Deinococcus radiodurans-derived extracellular vesicles remain unclear.
Aim: This study investigated the protective effects of membrane vesicles derived from D. radiodurans (R1-MVs) against H2O2-induced oxidative stress in HaCaT cells.
Results: R1-MVs were identified as 322 nm spherical molecules. Pretreatment with R1-MVs inhibited H2O2-mediated apoptosis in HaCaT cells by suppressing the loss of mitochondrial membrane potential and reactive oxygen species (ROS) production. R1-MVs increased the superoxide dismutase (SOD) and catalase (CAT) activities, restored glutathione (GSH) homeostasis, and reduced malondialdehyde (MDA) production in H2O2-exposed HaCaT cells. Moreover, the protective effect of R1-MVs against H2O2-induced oxidative stress in HaCaT cells was dependent on the downregulation of mitogen-activated protein kinase (MAPK) phosphorylation and the upregulation of the nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Furthermore, the weaker protective capabilities of R1-MVs derived from ΔDR2577 mutant than that of the wild-type R1-MVs confirmed our inferences and indicated that SlpA protein plays a crucial role in R1-MVs against H2O2-induced oxidative stress.
Conclusion: Taken together, R1-MVs exert significant protective effects against H2O2-induced oxidative stress in keratinocytes and have the potential to be applied in radiation-induced oxidative stress models.
期刊介绍：
iological Procedures Online publishes articles that improve access to techniques and methods in the medical and biological sciences.
We are also interested in short but important research discoveries, such as new animal disease models.
Topics of interest include, but are not limited to:
Reports of new research techniques and applications of existing techniques
Technical analyses of research techniques and published reports
Validity analyses of research methods and approaches to judging the validity of research reports
Application of common research methods
Reviews of existing techniques
Novel/important product information
Biological Procedures Online places emphasis on multidisciplinary approaches that integrate methodologies from medicine, biology, chemistry, imaging, engineering, bioinformatics, computer science, and systems analysis.
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