脂质体- fe3o4 -阿霉素介导的黑色素瘤治疗。

IF 3.1 Q2 PHARMACOLOGY & PHARMACY
Azalia Azlegini, Sirus Javadpour, Mohamad Ebrahim Bahrololoom
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引用次数: 1

摘要

目的:磁热疗是一种基于磁纳米颗粒(MNPs)涡流、磁滞和弛豫机制的治疗方法。像fe3o4这样的MNPs能够在交变磁场下产生热量。热敏脂质体(Lip)通过MNPs产生的热量从脂质层转化为液体层,并释放药物。方法:对不同组的阿霉素(DOX)、MNPs和脂质体进行评价。采用共沉淀法合成MNPs。利用蒸发器旋转技术将MNPs、DOX以及MNPs和DOX的组合有效地装载到脂质体中。研究了脂质体的磁性、微观结构、比吸收率(SAR)、zeta电位、MNPs的负载率和DOX浓度,以及脂质体的体外药物释放情况。最后,评估各组携带黑色素瘤的C57BL/6J小鼠的癌细胞坏死百分比。结果:脂质体中MNPs的负载率为18.52%,DOX的浓度为65%。Lip-DOX-MNPs在缓冲柠檬酸溶液中,当溶液温度在5分钟内达到42°C时,表现出很高的SAR。DOX的释放以ph依赖的方式发生。与其他治疗组相比,含有MNPs的治疗组的肿瘤体积显著减少。数值分析表明,Lip-MNPs-DOX小鼠的肿瘤体积为对照组的9.29%,肿瘤切片组织学检查显示70%坏死。结论:Lip-DOX-MNPs可作为抑制皮肤恶性肿瘤生长、促进肿瘤细胞坏死的有效药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Liposome-Fe<sub>3</sub> O<sub>4</sub>-Doxorubicin Mediated Treatment of Melanoma Tumors.

Liposome-Fe<sub>3</sub> O<sub>4</sub>-Doxorubicin Mediated Treatment of Melanoma Tumors.

Liposome-Fe<sub>3</sub> O<sub>4</sub>-Doxorubicin Mediated Treatment of Melanoma Tumors.

Liposome-Fe3 O4-Doxorubicin Mediated Treatment of Melanoma Tumors.

Purpose: Magnetic hyperthermia is a treatment method based on eddy currents, hysteresis, and relaxation mechanisms of magnetic nanoparticles (MNPs). MNPs such as Fe3 O4 have the ability to generate heat under an alternating magnetic field. Heat sensitive liposomes (Lip) convert from lipid layer to liquid layer through heat generated by MNPs and can release drugs. Methods: In this study, different groups of doxorubicin (DOX), MNPs and liposomes were evaluated. The MNPs were synthesized by co-precipitation method. The MNPs, DOX and a combination of MNPs and DOX were efficiently loaded into the liposomes using the evaporator rotary technique. Magnetic properties, microstructure, specific absorption rate (SAR), zeta potential, loading percentage of the MNPs and DOX concentration in liposomes, in vitro drug release of liposomes were studied. Finally, the necrosis percentage of cancer cells in C57BL/6J mice bearing melanoma tumors was assessed for all groups. Results: The loading percentages of MNPs and concentration of DOX in the liposomes were 18.52 and 65% respectively. The Lip-DOX-MNPs at the buffer citrate solution, showed highly SAR as the solution temperature reached 42°C in 5 minutes. The release of DOX occurred in a pH-dependent manner. The volume of tumor in the therapeutic groups containing the MNPs significantly decreased compared to the others. Numerical analysis showed that the tumor volume in mice receiving Lip-MNPs-DOX was 9.29% that of the control and a histological examination of the tumor section showed 70% necrosis. Conclusion: The Lip-DOX-MNPs could be effective agents which reduce malignant skin tumors growth and increase cancer cell necrosis.

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来源期刊
Advanced pharmaceutical bulletin
Advanced pharmaceutical bulletin PHARMACOLOGY & PHARMACY-
CiteScore
6.80
自引率
2.80%
发文量
51
审稿时长
12 weeks
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