利用质显微成像技术可视化小麦叶片中偶氮嘧菌酯的渗透。

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Soichiro Ikuta, Eiichiro Fukusaki, Shuichi Shimma
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引用次数: 0

摘要

杀菌剂必须渗透到植物的内部组织才能杀死致病真菌。质谱已被用来证实这种渗透,但由于提取步骤的原因,传统的质谱方法无法区分不同内部组织中的杀菌剂。然而,基质辅助激光解吸/电离质谱成像(MALDI-MSI)可以通过直接分析样品表面来检测杀菌剂对叶片的渗透。因此,本研究的目的是建立一种利用MALDI-MSI可视化小麦叶片横截面杀菌剂渗透的方法。观察到氮嘧菌酯从表皮渗透到叶片内部组织。此外,偶氮氧嘧啶在维管束周围的细胞中积累。本研究表明,MSI可用于评价杀菌剂在植物叶片中的渗透。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Visualization of azoxystrobin penetration in wheat leaves using mass microscopy imaging.

Visualization of azoxystrobin penetration in wheat leaves using mass microscopy imaging.

Visualization of azoxystrobin penetration in wheat leaves using mass microscopy imaging.

Visualization of azoxystrobin penetration in wheat leaves using mass microscopy imaging.

Fungicides must penetrate the internal tissues of plants to kill pathogenic fungi. Mass spectrometers have been used to confirm this penetration, but conventional mass spectrometric methods cannot distinguish the fungicides in different internal tissues owing to the extraction steps. However, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can detect the penetration of fungicides into leaf sections through direct analysis of the sample surfaces. Therefore, the objective of this study was to establish a method for visualizing fungicide penetration in wheat leaf cross sections using MALDI-MSI. The penetration of azoxystrobin from the epidermal to the internal tissue of the leaves was observed. Moreover, azoxystrobin accumulates in the cells around the vascular bundle. This study suggests that MSI can be useful for the evaluation of fungicide penetration in plant leaves.

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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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