Arti Ralta, Ajay Prakash, Praveen Kumar M, Rohit Kumar, Phulen Sarma, Alka Bhatia, Bikash Medhi, Amitava Chakrabarti
{"title":"南蛇藤种子提取物对癫痫和癫痫相关认知缺陷的神经保护作用。","authors":"Arti Ralta, Ajay Prakash, Praveen Kumar M, Rohit Kumar, Phulen Sarma, Alka Bhatia, Bikash Medhi, Amitava Chakrabarti","doi":"10.32598/bcn.2021.3154.1","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of <i>Celastrus paniculatus</i> seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model.</p><p><strong>Methods: </strong>PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, <i>C. paniculatus</i> 500 mg\\kg, pergolide 2 mg/kg, C. paniculatus (250 mg\\kg)+ Pergolide (1 mg/kg), and <i>C. paniculatus</i> (250 mg\\kg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin & Eosin (H&E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed.</p><p><strong>Results: </strong><i>C. paniculatus</i> (500 mg/kg) alone and in combination (<i>C. paniculatus</i> (250 mg\\ kg)+ pergolide (1 mg/kg) and <i>C. paniculatus</i> (250 mg\\kg)+ SVA (100 mg/kg)) significantly (P<0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. <i>C. paniculatus</i> and its combination also significantly (P<0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H&E staining.</p><p><strong>Conclusion: </strong><i>C. paniculatus</i> alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits.</p>","PeriodicalId":8701,"journal":{"name":"Basic and Clinical Neuroscience","volume":"14 1","pages":"155-166"},"PeriodicalIF":1.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/3d/BCN-14-155.PMC10279989.pdf","citationCount":"0","resultStr":"{\"title\":\"Neuroprotective Effect of <i>Celastrus Paniculatus</i> Seed Extract on Epilepsy and Epilepsy-associated Cognitive Deficits.\",\"authors\":\"Arti Ralta, Ajay Prakash, Praveen Kumar M, Rohit Kumar, Phulen Sarma, Alka Bhatia, Bikash Medhi, Amitava Chakrabarti\",\"doi\":\"10.32598/bcn.2021.3154.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of <i>Celastrus paniculatus</i> seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model.</p><p><strong>Methods: </strong>PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, <i>C. paniculatus</i> 500 mg\\\\kg, pergolide 2 mg/kg, C. paniculatus (250 mg\\\\kg)+ Pergolide (1 mg/kg), and <i>C. paniculatus</i> (250 mg\\\\kg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin & Eosin (H&E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed.</p><p><strong>Results: </strong><i>C. paniculatus</i> (500 mg/kg) alone and in combination (<i>C. paniculatus</i> (250 mg\\\\ kg)+ pergolide (1 mg/kg) and <i>C. paniculatus</i> (250 mg\\\\kg)+ SVA (100 mg/kg)) significantly (P<0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. <i>C. paniculatus</i> and its combination also significantly (P<0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H&E staining.</p><p><strong>Conclusion: </strong><i>C. paniculatus</i> alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits.</p>\",\"PeriodicalId\":8701,\"journal\":{\"name\":\"Basic and Clinical Neuroscience\",\"volume\":\"14 1\",\"pages\":\"155-166\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/3d/BCN-14-155.PMC10279989.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Basic and Clinical Neuroscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32598/bcn.2021.3154.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and Clinical Neuroscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32598/bcn.2021.3154.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
Neuroprotective Effect of Celastrus Paniculatus Seed Extract on Epilepsy and Epilepsy-associated Cognitive Deficits.
Introduction: Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of Celastrus paniculatus seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model.
Methods: PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, C. paniculatus 500 mg\kg, pergolide 2 mg/kg, C. paniculatus (250 mg\kg)+ Pergolide (1 mg/kg), and C. paniculatus (250 mg\kg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin & Eosin (H&E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed.
Results: C. paniculatus (500 mg/kg) alone and in combination (C. paniculatus (250 mg\ kg)+ pergolide (1 mg/kg) and C. paniculatus (250 mg\kg)+ SVA (100 mg/kg)) significantly (P<0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. C. paniculatus and its combination also significantly (P<0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H&E staining.
Conclusion: C. paniculatus alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits.
期刊介绍：
BCN is an international multidisciplinary journal that publishes editorials, original full-length research articles, short communications, reviews, methodological papers, commentaries, perspectives and “news and reports” in the broad fields of developmental, molecular, cellular, system, computational, behavioral, cognitive, and clinical neuroscience. No area in the neural related sciences is excluded from consideration, although priority is given to studies that provide applied insights into the functioning of the nervous system. BCN aims to advance our understanding of organization and function of the nervous system in health and disease, thereby improving the diagnosis and treatment of neural-related disorders. Manuscripts submitted to BCN should describe novel results generated by experiments that were guided by clearly defined aims or hypotheses. BCN aims to provide serious ties in interdisciplinary communication, accessibility to a broad readership inside Iran and the region and also in all other international academic sites, effective peer review process, and independence from all possible non-scientific interests. BCN also tries to empower national, regional and international collaborative networks in the field of neuroscience in Iran, Middle East, Central Asia and North Africa and to be the voice of the Iranian and regional neuroscience community in the world of neuroscientists. In this way, the journal encourages submission of editorials, review papers, commentaries, methodological notes and perspectives that address this scope.
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