{"title":"从艾蒿提取物中分离出的 C17H31NO15 对从玉米中分离出的黑曲霉的抗真菌作用研究","authors":"Basma Hamdy Amin, Nahed Mohammed Ayyat, Reyad Mohamed El-Sharkawy, Asmaa Mohamed Hafez","doi":"10.2174/2772434418666230627141639","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.</p><p><strong>Objective: </strong>Investigation of antifungal impact of various fractions of methanol extract of <i>Artemisia herba-alba</i> to <i>Aspergillus niger</i> as a plant pathogen.</p><p><strong>Methods: </strong><i>Artemisia herba-alba</i> extract was purified using column chromatography, giving various antifungal fractions tested versus <i>A. niger</i>.</p><p><strong>Results: </strong>The 6<sup>th</sup> fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, <sup>1</sup>HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated <i>A. niger</i> was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.</p><p><strong>Conclusion: </strong>These results revealed the possibility of using <i>Artemisia herba-alba</i> methanol extract as a promising antifungal <i>versus</i> phytopathogenic fungi, especially <i>A. niger</i> after more verification of results.</p>","PeriodicalId":74643,"journal":{"name":"Recent advances in anti-infective drug discovery","volume":" ","pages":"159-172"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of Antifungal Action of Fractions C<sub>17</sub>H<sub>31</sub>NO<sub>15</sub> Isolated from <i>Artemisia herba-alba</i> extract <i>versus</i> Isolated <i>Aspergillus niger</i> from Zee maize.\",\"authors\":\"Basma Hamdy Amin, Nahed Mohammed Ayyat, Reyad Mohamed El-Sharkawy, Asmaa Mohamed Hafez\",\"doi\":\"10.2174/2772434418666230627141639\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.</p><p><strong>Objective: </strong>Investigation of antifungal impact of various fractions of methanol extract of <i>Artemisia herba-alba</i> to <i>Aspergillus niger</i> as a plant pathogen.</p><p><strong>Methods: </strong><i>Artemisia herba-alba</i> extract was purified using column chromatography, giving various antifungal fractions tested versus <i>A. niger</i>.</p><p><strong>Results: </strong>The 6<sup>th</sup> fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, <sup>1</sup>HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated <i>A. niger</i> was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.</p><p><strong>Conclusion: </strong>These results revealed the possibility of using <i>Artemisia herba-alba</i> methanol extract as a promising antifungal <i>versus</i> phytopathogenic fungi, especially <i>A. niger</i> after more verification of results.</p>\",\"PeriodicalId\":74643,\"journal\":{\"name\":\"Recent advances in anti-infective drug discovery\",\"volume\":\" \",\"pages\":\"159-172\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Recent advances in anti-infective drug discovery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/2772434418666230627141639\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Recent advances in anti-infective drug discovery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/2772434418666230627141639","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigation of Antifungal Action of Fractions C17H31NO15 Isolated from Artemisia herba-alba extract versus Isolated Aspergillus niger from Zee maize.
Background: Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.
Objective: Investigation of antifungal impact of various fractions of methanol extract of Artemisia herba-alba to Aspergillus niger as a plant pathogen.
Methods: Artemisia herba-alba extract was purified using column chromatography, giving various antifungal fractions tested versus A. niger.
Results: The 6th fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, 1HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated A. niger was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.
Conclusion: These results revealed the possibility of using Artemisia herba-alba methanol extract as a promising antifungal versus phytopathogenic fungi, especially A. niger after more verification of results.
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