基于探针的定量PCR和RPA-Cas12a分子诊断检测番茄害虫绝对灰蛾(鳞翅目:gechiida)

IF 2.2 2区农林科学 Q1 ENTOMOLOGY

Journal of Economic Entomology Pub Date : 2023-06-13 DOI:10.1093/jee/toad079

Kyle M Lewald, Wenqi Song, Daniel Eweis-LaBolle, Cindy Truong, Kristine E Godfrey, Joanna C Chiu

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引用次数: 0

摘要

番茄害虫Phthorimaea absoluta Meyrick是高度侵入性的，但尚未入侵北美。然而，几个形态相似的物种已经存在，使得绝对假单胞菌的存在和入侵的检测具有挑战性。我们设计了一种定量PCR分子诊断方法来区分绝对P.， P. operculella (Zeller)，或Keiferia lycopersicella (Walsingham)(鳞翅目:Gelechiidae)的DNA。此外，我们开发了一种RPA-Cas12a分子诊断方法，允许对绝对假单胞菌DNA进行等温检测，从而消除了对热循环器的需要。RPA-Cas12a诊断结果可以简单地使用紫外光源和手机摄像头进行可视化。我们期望这些诊断能够改善检疫和预防措施，以应对这一严重的农业威胁。

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Probe-based quantitative PCR and RPA-Cas12a molecular diagnostics for detection of the tomato pest Phthorimaea absoluta (Lepidoptera: Gelechiidae).

The tomato pest Phthorimaea absoluta Meyrick is highly invasive but has not yet invaded North America. However, several morphologically similar species are already present, making detection of P. absoluta presence and invasion challenging. We designed a quantitative PCR molecular diagnostic to differentiate P. absoluta, P. operculella (Zeller), or Keiferia lycopersicella (Walsingham) (Lepidoptera: Gelechiidae) DNA. Additionally, we developed an RPA-Cas12a molecular diagnostic that allows for the isothermal detection of P. absoluta DNA, eliminating the need for a thermocycler. The results of the RPA-Cas12a diagnostic can be visualized simply using a UV light source and cell phone camera. We expect these diagnostics to improve quarantine and prevention measures against this serious agricultural threat.

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来源期刊

Journal of Economic Entomology 生物-昆虫学

CiteScore

4.60

自引率

9.10%

发文量

198

审稿时长

3-6 weeks

期刊介绍： Journal of Economic Entomology the most-cited entomological journal – publishes articles on the economic significance of insects and other arthropods and includes sections on apiculture & social insects, insecticides, biological control, household & structural insects, crop protection, forest entomology, and more. In addition to research papers, Journal of Economic Entomology publishes Reviews, interpretive articles in a Forum section, Short Communications, and Letters to the Editor. The journal is published bimonthly in February, April, June, August, October, and December.