利用重组植物产生的蛋白质建立严重急性呼吸系统综合征冠状病毒2型中和抗体检测方法

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2023-06-01 DOI:10.1016/j.btre.2023.e00796

Perawat Jirarojwattana , Balamurugan Shanmugaraj , Kaewta Rattanapisit , Waranyoo Phoolcharoen

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引用次数: 1

摘要

检测对严重急性呼吸系统综合征冠状病毒2型的免疫力对于评估疫苗反应和自然感染至关重要，但常规病毒中和试验（cVNT）需要BSL3和活病毒，而伪病毒中和试验需要专业设备和训练有素的专业人员。替代病毒中和试验（sVNT）是为了克服这些限制而开发的。本研究探讨了利用本氏烟草产生的血管紧张素转换酶2（ACE2）开发一种负担得起的中和抗体检测方法。结果表明，植物产生的ACE2可以与严重急性呼吸系统综合征冠状病毒2型的受体结合结构域（RBD）结合，并用于与植物产生的RBD蛋白一起开发sVNT。使用植物产生的蛋白质开发的sVNT在用30份RBD接种的小鼠血清进行验证时显示出高灵敏度和特异性，结果与cVNT滴度相关。这一初步发现表明，这些工厂可以为生产诊断试剂提供一个具有成本效益的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins

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Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins

Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme 2 (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2 can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with plant-produced RBD protein. The sVNT developed using plant-produced proteins showed high sensitivity and specificity when validated with a group of 30 RBD vaccinated mice sera and the results were correlated with cVNT titer. This preliminary finding suggests that the plants could offer a cost-effective platform for producing diagnostic reagents.

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.