胆固醇及其生物合成前体在膜组织和动力学中的作用:荧光方法。

IF 2.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sandeep Shrivastava, Yamuna Devi Paila, Amitabha Chattopadhyay
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引用次数: 0

摘要

胆固醇是高等真核生物膜中最具代表性的甾醇,是一个漫长而多步骤的生物合成途径的最终产物。肝甾醇和酶甾醇分别是Kandutsch-Russell和Bloch途径中胆固醇的生物合成前体。肝甾醇与胆固醇的区别仅仅在于固醇环中双键的位置,而酶甾醇与胆固醇的区别在于双键的位置和数量。在这项工作中,我们监测了胆固醇及其生物合成前体(胆甾醇和酶甾醇)对流体和凝胶相膜的膜组织和动力学的影响。为了实现这一目标,我们利用了两种荧光膜探针,DPH及其阳离子衍生物TMA-DPH。我们使用这些探针的结果表明,在液相POPC膜中,胆固醇及其生物合成前体(胆甾醇和酶甾醇)在维持膜组织和动力学(荧光各向异性和表观旋转相关时间)方面表现出相似的趋势。值得注意的是,尽管胆甾醇和酶甾醇在维持膜组织和动力学方面表现出相似的趋势,但胆固醇在凝胶相DPPC膜中相应的变化是不同的。这些结果表明,甾醇中双键的位置和数量是维持膜物理性质的重要决定因素。我们的研究结果具有重要意义,因为据报道,胆固醇前体的积累与严重的病理状况有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Role of Cholesterol and its Biosynthetic Precursors on Membrane Organization and Dynamics: A Fluorescence Approach.

Role of Cholesterol and its Biosynthetic Precursors on Membrane Organization and Dynamics: A Fluorescence Approach.

Cholesterol is the most representative sterol present in membranes of higher eukaryotes, and is the end product of a long and multistep biosynthetic pathway. Lathosterol and zymosterol are biosynthetic precursors of cholesterol in Kandutsch-Russell and Bloch pathways, respectively. Lathosterol differs with cholesterol merely in the position of the double bond in the sterol ring, whereas zymosterol differs with cholesterol in position and number of double bonds. In this work, we have monitored the effect of cholesterol and its biosynthetic precursors (lathosterol and zymosterol) on membrane organization and dynamics in fluid and gel phase membranes. Toward this goal, we have utilized two fluorescent membrane probes, DPH and its cationic derivative TMA-DPH. Our results using these probes show that cholesterol and its biosynthetic precursors (lathosterol and zymosterol) exhibit similar trend in maintaining membrane organization and dynamics (as reported by fluorescence anisotropy and apparent rotational correlation time), in fluid phase POPC membranes. Notably, although lathosterol and zymosterol show similar trend in maintaining membrane organization and dynamics, the corresponding change for cholesterol is different in gel phase DPPC membranes. These results demonstrate that the position and number of double bonds in sterols is an important determinant in maintaining membrane physical properties. Our results assume significance since accumulation of precursors of cholesterol have been reported to be associated with severe pathological conditions.

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来源期刊
Journal of Membrane Biology
Journal of Membrane Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
4.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.
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