鉴定大鼠大唾液腺主要排泄管中的干细胞:商业抗体的冒险。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Robert S Redman, José C Alvarez-Martinez
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引用次数: 1

摘要

我们研究了成熟实验室大鼠主要舌下、腮腺和下颌下唾液腺的主要排泄管(MED)的整个长度,以检测粘膜(杯状)和腔纤毛细胞、细胞增殖、凋亡的生物标志物和五种干细胞的生物标志物。脾和睾丸作为阳性对照。我们使用福尔马林固定,石蜡包埋组织。苏木精染色、伊红染色、阿利新蓝染色、周期性酸-希夫染色均未见粘膜细胞或管腔纤毛细胞。利用兔抗大鼠抗体进行免疫组化,与裂解的caspase-3、Ki-67、增殖活性和Sox 2产生异常反应。抗原回收后,无一抗和所有三个阴性对照,标记的巨噬细胞出现在脾脏。TUNEL染色显示每个切片有少量细胞发生凋亡。细胞角蛋白-5和c-Kit与干细胞抗原1 (Sca-1)在MED中被认为有效的反应主要发生在腺体和中间节段。除腺泡细胞和肌上皮细胞外,其他导管也被c-Kit和Sca-1染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identifying stem cells in the main excretory ducts of rat major salivary glands: adventures with commercial antibodies.

We investigated the entire length of the main excretory ducts (MED) of the major sublingual, parotid and submandibular salivary glands of mature laboratory rats for mucous (goblet) and luminal ciliated cells, biomarkers of cell proliferation, apoptosis, and five biomarkers of stem cells. Spleen and testis were used as positive controls. We used formalin fixed, paraffin embedded tissues. No mucous cells or cells with luminal cilia were observed in hematoxylin and eosin, alcian blue or periodic acid-Schiff stained sections. Immunohistochemistry using rabbit anti-rat antibodies produced anomalous reactions with cleaved caspase-3 for apoptosis, Ki-67 for proliferative activity and Sox 2. Following antigen retrieval, no primary antibody and all three negative controls, labeled macrophages appeared in the spleen. TUNEL staining revealed a few cells per section undergoing apoptosis. Reactions deemed valid occurred in MED with cytokeratin-5 and c-Kit and stem cell antigen 1 (Sca-1) mostly in the gland and middle segments. Other ducts, but not acini or myoepithelial cells, also were variably stained with c-Kit and Sca-1.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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