北冰洋沉积物微生物群的结构和功能潜力。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
William Kurdy, Galina Yakovleva, Olga Ilinskaya
{"title":"北冰洋沉积物微生物群的结构和功能潜力。","authors":"William Kurdy,&nbsp;Galina Yakovleva,&nbsp;Olga Ilinskaya","doi":"10.2323/jgam.2022.10.001","DOIUrl":null,"url":null,"abstract":"<p><p>Arctic ecosystems are affected by negative influence of climate change, pollution, and overexploitation of resources. Microorganisms playing a key role in preserving extreme econiches are poorly studied and require the use of modern methods for studying both their biodiversity and physiological activity. We applied Illumina MiSeq to the high-throughput 16S rRNA sequencing study of four Laptev Sea sediments from 64 - 185 m depth, using next generation sequencing enables rapid analysis of composition and diversity of prokaryotic communities. Although the dominant phylum in all samples was Proteobacteria, only the deepest sample contained a high number of archaeal organisms (19%) with the predominance of Methanosarcinaceace family in comparison with less 1% in the other three samples. This deepest sample had the lowest biodiversity and richness indices. Comparison of functional profiles of communities using Global Mapper tool revealed similar average abundance of infectiousness, drug resistance and environmental adaptation determinants in all samples, and high functional abundance for xenobiotic degradation in two samples. Among cultivated bacteria which could be promising producers of secreted RNase the representatives of Bacillus and Lysinibacillus genera were found. Our results contribute to improve our understanding of richness and ecological role of Laptev Sea microbiota.</p>","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"69 1","pages":"24-33"},"PeriodicalIF":0.8000,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Structure and Functional Potential of Arctic Sea Sediment Microbiota.\",\"authors\":\"William Kurdy,&nbsp;Galina Yakovleva,&nbsp;Olga Ilinskaya\",\"doi\":\"10.2323/jgam.2022.10.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Arctic ecosystems are affected by negative influence of climate change, pollution, and overexploitation of resources. Microorganisms playing a key role in preserving extreme econiches are poorly studied and require the use of modern methods for studying both their biodiversity and physiological activity. We applied Illumina MiSeq to the high-throughput 16S rRNA sequencing study of four Laptev Sea sediments from 64 - 185 m depth, using next generation sequencing enables rapid analysis of composition and diversity of prokaryotic communities. Although the dominant phylum in all samples was Proteobacteria, only the deepest sample contained a high number of archaeal organisms (19%) with the predominance of Methanosarcinaceace family in comparison with less 1% in the other three samples. This deepest sample had the lowest biodiversity and richness indices. Comparison of functional profiles of communities using Global Mapper tool revealed similar average abundance of infectiousness, drug resistance and environmental adaptation determinants in all samples, and high functional abundance for xenobiotic degradation in two samples. Among cultivated bacteria which could be promising producers of secreted RNase the representatives of Bacillus and Lysinibacillus genera were found. Our results contribute to improve our understanding of richness and ecological role of Laptev Sea microbiota.</p>\",\"PeriodicalId\":15842,\"journal\":{\"name\":\"Journal of General and Applied Microbiology\",\"volume\":\"69 1\",\"pages\":\"24-33\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of General and Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.2323/jgam.2022.10.001\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of General and Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2323/jgam.2022.10.001","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

北极生态系统受到气候变化、污染和资源过度开发的负面影响。微生物在保护极端生态系统中发挥着关键作用,但研究很少,需要使用现代方法来研究它们的生物多样性和生理活性。我们使用Illumina MiSeq对4个Laptev海沉积物进行了高通量16S rRNA测序研究,深度为64 - 185 m,使用下一代测序技术可以快速分析原核生物群落的组成和多样性。虽然所有样品中的优势门都是变形菌门,但只有最深的样品中含有大量的古细菌生物(19%),其中Methanosarcinaceace家族占优势,而其他三个样品的优势门不到1%。最深的样本生物多样性和丰富度指数最低。使用Global Mapper工具比较群落的功能谱显示,所有样本中传染性、耐药性和环境适应决定因素的平均丰度相似,两个样本中外源性降解的功能丰度很高。在有可能产生分泌rna酶的培养菌中,发现了芽孢杆菌属和赖氨酸芽孢杆菌属的代表菌。这些结果有助于提高我们对拉普捷夫海微生物群丰富度和生态作用的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structure and Functional Potential of Arctic Sea Sediment Microbiota.

Arctic ecosystems are affected by negative influence of climate change, pollution, and overexploitation of resources. Microorganisms playing a key role in preserving extreme econiches are poorly studied and require the use of modern methods for studying both their biodiversity and physiological activity. We applied Illumina MiSeq to the high-throughput 16S rRNA sequencing study of four Laptev Sea sediments from 64 - 185 m depth, using next generation sequencing enables rapid analysis of composition and diversity of prokaryotic communities. Although the dominant phylum in all samples was Proteobacteria, only the deepest sample contained a high number of archaeal organisms (19%) with the predominance of Methanosarcinaceace family in comparison with less 1% in the other three samples. This deepest sample had the lowest biodiversity and richness indices. Comparison of functional profiles of communities using Global Mapper tool revealed similar average abundance of infectiousness, drug resistance and environmental adaptation determinants in all samples, and high functional abundance for xenobiotic degradation in two samples. Among cultivated bacteria which could be promising producers of secreted RNase the representatives of Bacillus and Lysinibacillus genera were found. Our results contribute to improve our understanding of richness and ecological role of Laptev Sea microbiota.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信