芍药苷对大鼠肠易激综合征的治疗作用

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Lei Wang, Jinyan Lei, Zeyu Zhao, Jianwei Jia, Li Wang
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引用次数: 0

摘要

背景:肠易激综合征(IBS)是一种功能性肠病(FBD):肠易激综合征(IBS)是一种功能性肠病(FBD):评估芍药苷(PF)对大鼠肠易激综合征的治疗效果:方法:将60只雄性Sprague-Dawley大鼠随机分为正常组、模型组、药物阳性组、低剂量芍药苷组、中剂量芍药苷组和高剂量芍药苷组(n = 10)。连续灌胃 2 周后,根据腹部退缩反射(AWR)评分、粪便含水量和结肠组织病理变化评估 PF 对腹痛症状的影响。通过酶联免疫吸附试验检测 D-乳酸盐、白细胞介素-1β(IL-1β)、转化生长因子-β(TGF-β)和肿瘤坏死因子-α(TNF-α),通过 Western 印迹检测磷酸化核因子卡巴 B(p-NF-κB)p65。采用 16S 核糖体 RNA 测序法探讨了肠道菌群的丰度和多样性变化:结果:PF组结肠粘膜形态完整,腺体排列整齐,结构清晰,无明显炎性细胞浸润。与模型组相比,PF组的痛阈值、闭塞带-1(ZO-1)和闭塞素的mRNA和蛋白水平明显升高,20 mmHg压力下的AWR评分、粪便含水量、IL-1β、TGF-β和TNF-α的mRNA水平、p-NF-κB p65的蛋白水平和血清D-乳酸盐水平降低,血清IL-1β、TGF-β和TNF-α的水平降低(p < 0.05, p < 0.01).与模型组相比,PF组的乳酸杆菌、阿克曼斯菌、阿利斯蒂普斯菌和嗜酸乳杆菌的含量较高,但脱硫弧菌、副菌和肠球菌的含量较低:结论:PF 对大鼠肠易激综合征的治疗作用可能是通过调节肠道菌群,然后上调结肠组织中 ZO-1 和闭塞素的表达,同时下调 IL-1β、TGF-β、TNF-α、D-乳酸盐和 p-NF-κB p65 的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats.

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats.

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats.

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats.

Background: Irritable bowel syndrome (IBS) is a functional bowel disorder (FBD).

Objectives: To assess the therapeutic effects of paeoniflorin (PF) on IBS in rats.

Method: Sixty male Sprague-Dawley rats were randomly divided into normal, model, positive drug, low-dose PF, medium-dose PF and high-dose PF groups (n = 10). After gavage for 2 consecutive weeks, the effect of PF on abdominal pain symptoms was assessed based on the abdominal withdrawal reflex (AWR) score, fecal water content and pathological changes in colon tissues. D-lactate, interleukin-1β (IL-1β), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay, and phosphorylated nuclear factor kappa B (p-NF-κB) p65 was detected by Western blotting. The abundance and diversity changes of intestinal flora were explored using 16S ribosomal RNA sequencing.

Result: In PF groups, the mucosal morphology of colon tissues was intact, and the glands were arranged neatly and structured clearly, without obvious inflammatory cell infiltration. Compared with the model group, PF groups had significantly elevated pain threshold, and mRNA and protein levels of zonula occludens-1 (ZO-1) and occludin, decreased AWR score at 20 mmHg pressure, fecal water content, mRNA levels of IL-1β, TGF-β, and TNF-α, protein level of p-NF-κB p65 and level of serum D-lactate, and reduced levels of serum IL-1β, TGF-β, and TNF-α (p < 0.05, p < 0.01). PF groups had higher abundance of Lactobacillus, Akkermansia, Alistipes, and Bacteroides, but lower abundance of Desulfovibrio, Parasutterella, and Enterococcus than those of the model group.

Conclusions: PF exerts therapeutic effects on IBS in rats probably by regulating the intestinal flora, and then up-regulating the expressions of ZO-1 and occludin in colon tissue while down-regulating the levels of IL-1β, TGF-β, TNF-α, D-lactate and p-NF-κB p65.

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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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