IncRNA MIAT通过miR-411-5p/JAG1轴加速瘢痕疙瘩形成。

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yingyan Yu, Yujie Dong, Benyuan Deng, Ting Yang
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引用次数: 0

摘要

长链非编码RNA (lncRNA)心肌梗死相关转录物(MIAT)调控多种细胞的生物学功能。本研究的目的是探讨MIAT的机制及其如何影响瘢痕疙瘩的进展。采用Western blot和定量逆转录聚合酶链式反应分析,定量检测瘢痕疙瘩组织和瘢痕疙瘩成纤维细胞(kelfibs)中MIAT、JAG1、miR-411-5p的表达。通过CCK-8、创面愈合和Transwell实验评估MIAT、JAG1和miR-411-5p对KEL FIBs增殖、迁移和侵袭能力的影响。为了确定MIAT、JAG1和miR-411-5p之间的结合关系,我们进行了荧光素酶报告基因和RIP实验。在瘢痕疙瘩组织和KEL FIBs中,MIAT和JAG1上调,miR-411-5p下调。下调MIAT或JAG1可显著抑制细胞增殖、迁移和侵袭。相反,抑制miR-411-5p的表达会产生相反的效果。至于机制,MIAT海绵miR-411-5p靶向JAG1。MIAT通过调节miR-411-5p/JAG1轴加速瘢痕疙瘩的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IncRNA MIAT Accelerates Keloid Formation by miR-411-5p/JAG1 Axis.

The long non-coding RNA (lncRNA) myocardial infarction-associated transcript (MIAT) regulates the biological functions of many kinds of cells. The aim of this study is to explore the mechanism of MIAT and how it affects keloid progression. The expressions of MIAT, JAG1, and miR-411-5p in keloid tissues and keloid fibroblasts (KEL FIBs) were quantified by conducting Western blot and quantitative reverse transcription polymerase chain reaction analyses. The influences of MIAT, JAG1, and miR-411-5p on the abilities of KEL FIBs to proliferate, migrate, and invade were assessed by means of the CCK-8, wound healing, and Transwell experiments. To determine the binding relationship among MIAT, JAG1, and miR-411-5p, we performed luciferase reporter and RIP experiments. In keloid tissues and KEL FIBs, MIAT and JAG1 were upregulated while miR-411-5p was downregulated. Knocking-down MIAT or JAG1 significantly inhibited proliferation, migration and invasion. On the contrary, suppressing miR-411-5p expression produced an opposite effect. With regard to mechanisms, MIAT sponged miR-411-5p, which targeted JAG1. MIAT accelerates keloid formation by modulating the miR-411-5p/JAG1 axis.

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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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