含和不含纳米银的生物陶瓷水泥对粪肠球菌的抗菌效果评价。

IF 1.1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
T P S Andrade, L S Barroso, M Amaral, L R Siliva-Concilio, C A Pinto, K Baroudi
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引用次数: 0

摘要

目的:比较纳米银与纳米银生物陶瓷牙髓胶结剂对粪肠球菌的抑菌效果。材料与方法:共分为6组(n=8),根据材料分为:MTA Flow + AgNP;MTA流组;生物C修复+ AgNP;生物修复;PBS Cimmo + AgNP;PBS Cimmo。各组在模拟体液粪肠球菌(Enterococcus faecalis, SBF)悬浮液中维持72 h。分别于0、24、48和72 h从悬浮液中取出100 μL的样品,在培养皿中接种3次。使用菌落计数器计数菌落形成单位(CFU)。所描述的所有程序都在层流室中进行。统计学分析采用双因素方差分析,采用Tukey事后检验和配对t检验(α = 0.05)。结果:纳米银的加入对MTA和CIMMO PBS水泥的生长有统计学意义(p⟨0.05),MTA组的细菌生长最低。各组间只有48 h和72 h的结果无差异。结论:纳米银颗粒加入生物陶瓷水泥中能有效促进细菌加速死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the Antimicrobial Effect on Enterococcus Faecalis of Bioceramic Cements with and without Silver Nanoparticles.

Objective: To compare the antimicrobial effect of bioceramic endodontic cements with and without silver nanoparticles on Enterococcus faecalis.

Materials and methods: Six groups were evaluated (n=8), and divided according to the materials: MTA Flow + AgNP ; MTA Flow Group; Bio C Repair + AgNP; Bio C Repair; PBS Cimmo + AgNP; PBS Cimmo. The groups were maintained for 72 h in the suspension of Enterococcus faecalis in Simulated Body Fluid (SBF). Samples of 100 μL were removed from the suspension at 0, 24, 48 and 72 h and seeded in triplicate in a Petri dish. Colony forming units (CFU) were counted using a colony counter. All procedures described were performed in a laminar flow chamber. Two-way ANOVA followed by Tukey's post hoc test and paired t-test were used for statistical analyses (α = 0.05).

Results: The addition of silver nanoparticles resulted in a statistically significant difference for MTA and CIMMO PBS cements (p⟨0.05), with the lowest bacterial growth being shown by the MTA group. For all groups, only the times of 48 and 72 h presented results without differences.

Conclusion: The addition of silver nanoparticles to bioceramic cements was efficient to promote an acceleration of bacterial death.

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来源期刊
CiteScore
2.30
自引率
7.70%
发文量
0
期刊介绍: The European Journal of Prosthodontics and Restorative Dentistry is published quarterly and includes clinical and research articles in subjects such as prosthodontics, operative dentistry, implantology, endodontics, periodontics and dental materials.
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