Nurul Najwa Ainaa Alias, Sharina Omar, Nur Indah Ahmad, Malaika Watanabe, Sun Tee Tay, Nor Azlina Aziz, Farina Mustaffa-Kamal
{"title":"马来西亚收容所猫巴尔通体感染及其相关危险因素分析","authors":"Nurul Najwa Ainaa Alias, Sharina Omar, Nur Indah Ahmad, Malaika Watanabe, Sun Tee Tay, Nor Azlina Aziz, Farina Mustaffa-Kamal","doi":"10.4142/jvs.22277","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Poor disease management and irregular vector control could predispose sheltered animals to disease such as feline <i>Bartonella</i> infection, a vector-borne zoonotic disease primarily caused by <i>Bartonella henselae</i>.</p><p><strong>Objectives: </strong>This study investigated the status of <i>Bartonella</i> infection in cats from eight (n = 8) shelters by molecular and serological approaches, profiling the CD4:CD8 ratio and the risk factors associated with <i>Bartonella</i> infection in shelter cats.</p><p><strong>Methods: </strong><i>Bartonella</i> deoxyribonucleic acid (DNA) was detected through polymerase chain reaction (PCR) targeting 16S-23S rRNA internal transcribed spacer gene, followed by DNA sequencing. <i>Bartonella</i> IgM and IgG antibody titre, CD4 and CD8 profiles were detected using indirect immunofluorescence assay and flow cytometric analysis, respectively.</p><p><strong>Results: </strong><i>B. henselae</i> was detected through PCR and sequencing in 1.0% (1/101) oral swab and 2.0% (1/50) cat fleas, while another 3/50 cat fleas carried <i>B. clarridgeiae.</i> Only 18/101 cats were seronegative against <i>B. henselae</i>, whereas 30.7% (31/101) cats were positive for both IgM and IgG, 8% (18/101) cats had IgM, and 33.7% (34/101) cats had IgG antibody only. None of the eight shelters sampled had <i>Bartonella</i> antibody-free cats. Although abnormal CD4:CD8 ratio was observed in 48/83 seropositive cats, flea infestation was the only significant risk factor observed in this study.</p><p><strong>Conclusions: </strong>The present study provides the first comparison on the <i>Bartonella</i> spp. antigen, antibody status and CD4:CD8 ratio among shelter cats. The high <i>B. henselae</i> seropositivity among shelter cats presumably due to significant flea infestation triggers an alarm of whether the infection could go undetectable and its potential transmission to humans.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b9/be/jvs-24-e38.PMC10244140.pdf","citationCount":"0","resultStr":"{\"title\":\"Profiling <i>Bartonella</i> infection and its associated risk factors in shelter cats in Malaysia.\",\"authors\":\"Nurul Najwa Ainaa Alias, Sharina Omar, Nur Indah Ahmad, Malaika Watanabe, Sun Tee Tay, Nor Azlina Aziz, Farina Mustaffa-Kamal\",\"doi\":\"10.4142/jvs.22277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Poor disease management and irregular vector control could predispose sheltered animals to disease such as feline <i>Bartonella</i> infection, a vector-borne zoonotic disease primarily caused by <i>Bartonella henselae</i>.</p><p><strong>Objectives: </strong>This study investigated the status of <i>Bartonella</i> infection in cats from eight (n = 8) shelters by molecular and serological approaches, profiling the CD4:CD8 ratio and the risk factors associated with <i>Bartonella</i> infection in shelter cats.</p><p><strong>Methods: </strong><i>Bartonella</i> deoxyribonucleic acid (DNA) was detected through polymerase chain reaction (PCR) targeting 16S-23S rRNA internal transcribed spacer gene, followed by DNA sequencing. <i>Bartonella</i> IgM and IgG antibody titre, CD4 and CD8 profiles were detected using indirect immunofluorescence assay and flow cytometric analysis, respectively.</p><p><strong>Results: </strong><i>B. henselae</i> was detected through PCR and sequencing in 1.0% (1/101) oral swab and 2.0% (1/50) cat fleas, while another 3/50 cat fleas carried <i>B. clarridgeiae.</i> Only 18/101 cats were seronegative against <i>B. henselae</i>, whereas 30.7% (31/101) cats were positive for both IgM and IgG, 8% (18/101) cats had IgM, and 33.7% (34/101) cats had IgG antibody only. None of the eight shelters sampled had <i>Bartonella</i> antibody-free cats. Although abnormal CD4:CD8 ratio was observed in 48/83 seropositive cats, flea infestation was the only significant risk factor observed in this study.</p><p><strong>Conclusions: </strong>The present study provides the first comparison on the <i>Bartonella</i> spp. antigen, antibody status and CD4:CD8 ratio among shelter cats. The high <i>B. henselae</i> seropositivity among shelter cats presumably due to significant flea infestation triggers an alarm of whether the infection could go undetectable and its potential transmission to humans.</p>\",\"PeriodicalId\":17557,\"journal\":{\"name\":\"Journal of Veterinary Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b9/be/jvs-24-e38.PMC10244140.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4142/jvs.22277\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.22277","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Profiling Bartonella infection and its associated risk factors in shelter cats in Malaysia.
Background: Poor disease management and irregular vector control could predispose sheltered animals to disease such as feline Bartonella infection, a vector-borne zoonotic disease primarily caused by Bartonella henselae.
Objectives: This study investigated the status of Bartonella infection in cats from eight (n = 8) shelters by molecular and serological approaches, profiling the CD4:CD8 ratio and the risk factors associated with Bartonella infection in shelter cats.
Methods: Bartonella deoxyribonucleic acid (DNA) was detected through polymerase chain reaction (PCR) targeting 16S-23S rRNA internal transcribed spacer gene, followed by DNA sequencing. Bartonella IgM and IgG antibody titre, CD4 and CD8 profiles were detected using indirect immunofluorescence assay and flow cytometric analysis, respectively.
Results: B. henselae was detected through PCR and sequencing in 1.0% (1/101) oral swab and 2.0% (1/50) cat fleas, while another 3/50 cat fleas carried B. clarridgeiae. Only 18/101 cats were seronegative against B. henselae, whereas 30.7% (31/101) cats were positive for both IgM and IgG, 8% (18/101) cats had IgM, and 33.7% (34/101) cats had IgG antibody only. None of the eight shelters sampled had Bartonella antibody-free cats. Although abnormal CD4:CD8 ratio was observed in 48/83 seropositive cats, flea infestation was the only significant risk factor observed in this study.
Conclusions: The present study provides the first comparison on the Bartonella spp. antigen, antibody status and CD4:CD8 ratio among shelter cats. The high B. henselae seropositivity among shelter cats presumably due to significant flea infestation triggers an alarm of whether the infection could go undetectable and its potential transmission to humans.
期刊介绍:
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.