小反刍兽疫病毒 V 蛋白的原核表达及间接 ELISA 的开发。

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-06-08 DOI:10.1080/10495398.2023.2221703

Guili Lu, Ping Wang, Shukui Miao, Jiong Huang, Wenge Ma, Xiaoyun Mi, Jing Xue, Kayizha Shayilan, Xueyun Yang, Genqiang Yan

{"title":"小反刍兽疫病毒 V 蛋白的原核表达及间接 ELISA 的开发。","authors":"Guili Lu, Ping Wang, Shukui Miao, Jiong Huang, Wenge Ma, Xiaoyun Mi, Jing Xue, Kayizha Shayilan, Xueyun Yang, Genqiang Yan","doi":"10.1080/10495398.2023.2221703","DOIUrl":null,"url":null,"abstract":"In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"5011-5015"},"PeriodicalIF":1.7000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA.\",\"authors\":\"Guili Lu, Ping Wang, Shukui Miao, Jiong Huang, Wenge Ma, Xiaoyun Mi, Jing Xue, Kayizha Shayilan, Xueyun Yang, Genqiang Yan\",\"doi\":\"10.1080/10495398.2023.2221703\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.\",\"PeriodicalId\":7836,\"journal\":{\"name\":\"Animal Biotechnology\",\"volume\":\" \",\"pages\":\"5011-5015\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/10495398.2023.2221703\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/6/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/10495398.2023.2221703","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/6/8 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

摘要

在这项研究中，我们重组表达了小反刍兽疫病毒（PPRV）的V蛋白，并使用间接酶联免疫吸附法（i-ELISA）评估了其对PPRV感染的诊断价值。在血清稀释度为 1:400 时，V 蛋白包被抗原的最佳浓度为 15 纳克/孔，最佳阳性阈值为 0.233。交叉反应试验表明，基于 V 蛋白的 i-ELISA对 PPRV具有特异性，且重现性一致，病毒中和试验的特异性为 82.6%，灵敏度为 100%。将重组 V 蛋白作为 ELISA 抗原可用于 PPRV 感染的血清流行病学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA.

In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes