{"title":"麻黄叶水醇提取物对环磷酰胺治疗雄性不育大鼠影响的实验研究。","authors":"Shiva Saleh, Aref Ghanaatpisheh, Hoda Haghshenas, Negar Parvin, Elmira Mikaeiliagah, Hossein Kargar Jahromi, Bahare Ebrahimi","doi":"10.18502/ijrm.v21i4.13268","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.</p><p><strong>Objective: </strong>This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.</p><p><strong>Results: </strong>While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p <math><mo><</mo></math> 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p <math><mo><</mo></math> 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p <math><mo><</mo></math> 0.05). Although CP's role on spermatogonia counts (57.5 <math><mo>±</mo></math> 5.2 in CP, 67.1 <math><mo>±</mo></math> 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.</p><p><strong>Conclusion: </strong>Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"285-294"},"PeriodicalIF":1.6000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227357/pdf/","citationCount":"0","resultStr":"{\"title\":\"The effect of leaf hydroalcoholic extract of Ephedra pachyclada infertility in male rats treated with cyclophosphamide: An experimental study.\",\"authors\":\"Shiva Saleh, Aref Ghanaatpisheh, Hoda Haghshenas, Negar Parvin, Elmira Mikaeiliagah, Hossein Kargar Jahromi, Bahare Ebrahimi\",\"doi\":\"10.18502/ijrm.v21i4.13268\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.</p><p><strong>Objective: </strong>This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.</p><p><strong>Results: </strong>While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p <math><mo><</mo></math> 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p <math><mo><</mo></math> 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p <math><mo><</mo></math> 0.05). Although CP's role on spermatogonia counts (57.5 <math><mo>±</mo></math> 5.2 in CP, 67.1 <math><mo>±</mo></math> 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.</p><p><strong>Conclusion: </strong>Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.</p>\",\"PeriodicalId\":14386,\"journal\":{\"name\":\"International Journal of Reproductive Biomedicine\",\"volume\":\"21 4\",\"pages\":\"285-294\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227357/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Reproductive Biomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijrm.v21i4.13268\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Reproductive Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijrm.v21i4.13268","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
The effect of leaf hydroalcoholic extract of Ephedra pachyclada infertility in male rats treated with cyclophosphamide: An experimental study.
Background: Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.
Objective: This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.
Materials and methods: In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29 day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.
Results: While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p 0.05). Although CP's role on spermatogonia counts (57.5 5.2 in CP, 67.1 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.
Conclusion: Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.
期刊介绍：
The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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