长非编码 RNA LPP-AS2 通过调控 miR-132-3p/OLFM1 轴在甲状腺癌中发挥抗肿瘤作用

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Bowei Zhang, Tong Liu, Yi Gu, Li Ren, Jinju Wang, Chao Feng, Zhe Song
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引用次数: 0

摘要

长非编码 RNA(lncRNA)LPP-AS2 在不同癌症中的促癌功能已被证实。然而,它在甲状腺癌(THCA)中的作用仍未确定。研究人员通过逆转录定量聚合酶链反应和 Western 印迹法估测了 lncRNA LPP-AS2、miR-132-3p 和 OLFM1 的表达。通过CCK8检测、Transwell侵袭检测、划痕伤口愈合迁移检测和caspase-3活性定量来评估THCA细胞的功能。体内试验也用于评估肿瘤生长。为了阐明 miR-132-3p 与 lncRNA LPP-AS2 和 OLFM1 的相互作用,还进行了荧光素酶报告实验和 RNA 免疫沉淀实验(RIPA)。THCA组织和细胞的lncRNA LPP-AS2和OLFM1表达较差,而miR-132-3p表达强劲。过表达lncRNA LPP-AS2会抑制THCA细胞的增殖、迁移和侵袭,并改善caspase-3的活性。miR-132-3p 与 lncRNA LPP-AS2 和 OLFM1 之间存在相互作用。从功能上讲,过表达 miR-132-3p 会促进 THCA 细胞的恶性表型。然而,额外的lncRNA LPP-AS2过表达则会消除这种肿瘤促进作用。体外实验还表明,OLFM1过表达对THCA细胞恶性作用的抑制作用可被miR-132-3p模拟物抵消。lncRNA LPP-AS2通过miR-132-3p/OLFM1轴阻碍THCA进展。我们的发现为干扰THCA的进展提供了一种潜在的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Long Non-Coding RNA LPP-AS2 Plays an Anti-Tumor Role in Thyroid Carcinoma by Regulating the miR-132-3p/OLFM1 Axis.

The cancer-promoting function of the long non-coding RNA (lncRNA) LPP-AS2 has been documented in different cancers. Nonetheless, its role in thyroid carcinoma (THCA) remains unestablished. Reverse transcription quantitative polymerase chain reaction and Western blotting were conducted to estimate the expressions of lncRNA LPP-AS2, miR-132-3p, and OLFM1. The THCA cells' functions were assessed through CCK8 assays, Transwell invasion assays, scratch wound-healing migration assays, and quantification of caspase-3 activity. The in vivo assays were also implemented to assess tumor growth. Luciferase reporter and RNA immuno-precipitation assay (RIPA) experiments were executed to elucidate the interactions of miR-132-3p with lncRNA LPP-AS2 and OLFM1. THCA tissues and cells exhibited poor lncRNA LPP-AS2 and OLFM1 expressions and a robust expression of miR-132-3p. Overexpressing lncRNA LPP-AS2 constrained THCA cell proliferation, migration, and invasion and improved caspase-3 activity. The anti-tumor function of lncRNA LPP-AS2 was also validated in vivo. miR-132-3p had an interplay with lncRNA LPP-AS2 and OLFM1. Functionally, overexpressing miR-132-3p promoted the malignant THCA cell phenotypes. However, that tumor promotion was abolished by the additional overexpression of lncRNA LPP-AS2. The in vitro experiments also demonstrated that the repressive effect of OLFM1 overexpression on THCA cell malignant action could be offset by the miR-132-3p mimic. lncRNA LPP-AS2 impedes THCA progression via the miR-132-3p/OLFM1 axis. Our findings contribute a potential strategy in interfering with THCA progression.

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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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