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引用次数: 0
摘要
虽然褪黑激素(MT)对植物非生物胁迫耐受性的改善作用已被广泛报道,但这一过程的机理仍不清楚。这项工作研究了 MT 提高辣椒(Capsicum annuum)植物耐旱性的机制。将 0.1mM MT 处理剂叶面喷洒在生长速度分别为 80% 和 40% 的植株上,持续 3 天。干旱胁迫导致植株干重、相对含水量、叶片水势、PSII 效率(F v /F m 比率)、叶绿素、可溶性蛋白质、叶片和根部氮含量显著下降。干旱增加了过氧化氢、丙二醛(MDA)、硝酸盐、铵、游离氨基酸、可溶性糖、脯氨酸和甘氨酸甜菜碱。干旱还增加了过氧化物酶(POD)、谷胱甘肽 S 转移酶(GST)和过氧化氢酶(CAT)的活性、电解质渗漏(EL)和甲基乙二醛(MG)。MT 预处理通过激活硝酸还原酶(NR)、亚硝酸还原酶(NiR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)和谷氨酰胺脱氢酶(GDH)等多种酶的活性,减少了氧化应激,改善了氮代谢。它还激活了与乙二醛酶系统有关的酶(Gly I 和 Gly II),降低了 NO3 - 、NH4 + 和游离氨基酸的含量。我们的研究提出了一种成本效益高且可持续的解决方案,通过提高植物生长、光合作用和氮含量,在限水条件下提高作物产量。
Melatonin improves drought stress tolerance of pepper (Capsicum annuum) plants via upregulating nitrogen metabolism.
While ameliorating effects of melatonin (MT) on abiotic stress tolerance in plants are widely reported, the mechanism that underlies this process remains elusive. This work investigated mechanisms by which MT improved drought tolerance in pepper (Capsicum annuum ) plants. A foliar spray of 0.1mM MT treatment was applied to plants grown at 80% and 40% of full field capacity for 3days. Drought stress caused a significant decrease in plant dry weight, relative water content, leaf water potential, PSII efficiency (F v /F m ratio), chlorophyll, soluble protein, leaf and root nitrogen content. Drought increased hydrogen peroxide, malondialdehyde (MDA), nitrate, ammonium, free amino acids, soluble sugars, proline and glycine betaine. Drought also increased peroxidase (POD), glutathione S-transferase (GST) and catalase (CAT) activities, electrolyte leakage (EL) and methylglyoxal (MG). MT pre-treatment reduced oxidative stress and improved nitrogen metabolism by activating various enzymes such as nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthetase (GOGAT) and glutamine dehydrogenase (GDH) activities. It also activated enzymes related to the glyoxalase system (Gly I and Gly II) and decreased NO3 - , NH4 + and free amino acid content. Our study suggests a cost-effective and sustainable solution to improve crop productivity in water-limited conditions, by enhancing plant growth, photosynthesis and nitrogen content.
期刊介绍:
Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance.
Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.